STK40 Mouse mAb: FAQs for Research Use

STK40 Mouse mAb is a monoclonal antibody raised in mouse, designed to specifically target the STK40 (Serine/Threonine Kinase 40) protein for use in a range of immunological and biochemical research applications.

Product Name

STK40 Mouse mAb

SKU / Catalog Number

Ab349965

Host Species

Mouse

Antibody Type

Monoclonal Antibody (mAb)

Target Protein

STK40 (Serine/Threonine Kinase 40)

Reference / OEM Code

absea#KC-36962

What Is STK40 and Why Is It Scientifically Relevant?

STK40 (Serine/Threonine Kinase 40) is a member of the serine/threonine protein kinase family. Serine/threonine kinases play fundamental roles in intracellular signal transduction, regulating processes such as cell proliferation, differentiation, apoptosis, and stress responses. STK40 has been reported in the literature to be involved in embryonic development and in modulating inflammatory signaling pathways. Reliable, specific antibodies such as STK40 Mouse mAb (Ab349965) are essential tools for researchers investigating STK40 expression, localization, and function in relevant biological systems.

Recommended Applications and Usage Guidelines

STK40 Mouse mAb (Ab349965) is intended for use in standard immunological detection applications. Common applications for monoclonal antibodies of this class typically include Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and Immunoprecipitation (IP). Users should consult the specific product datasheet for Ab349965 for confirmed and validated application details, recommended dilutions, and positive control information, as these parameters are determined empirically during antibody validation. Always titrate the antibody for your specific experimental system and sample type to achieve optimal signal-to-noise ratio.

Note: Specific validated applications, dilution ranges, and species reactivity for Ab349965 should be confirmed via the official product datasheet. The information above reflects general guidance for mouse monoclonal antibodies targeting intracellular kinases.

Storage, Handling, and Stability

Proper storage is critical to maintaining the activity and specificity of STK40 Mouse mAb. As a general best practice for monoclonal antibodies, the following guidelines apply unless the product datasheet specifies otherwise:

• Store at –20 °C for long-term storage.
• For short-term use (up to 1 month), storage at 4 °C is acceptable.
• Avoid repeated freeze-thaw cycles, which can lead to antibody aggregation and loss of activity. Aliquot the antibody upon first thaw.
• Keep the antibody away from direct light and contamination.
• Do not use beyond the expiry date stated on the vial label.

Please refer to the official Ab349965 datasheet for exact formulation, buffer composition, and storage conditions specific to this product.

Troubleshooting Common Experimental Issues

If you encounter unexpected results when using STK40 Mouse mAb, consider the following troubleshooting steps:

• No signal / weak signal: Check antibody concentration and dilution. Verify that the target protein is expressed in your cell or tissue sample. Ensure antigen retrieval conditions (for IHC/IF) are appropriate for the epitope. Confirm that secondary antibody is compatible with the mouse host species of this primary antibody.
• High background: Increase blocking time or use an alternative blocking agent. Optimize primary and secondary antibody dilutions. Ensure washing steps are thorough.
• Non-specific bands (WB): Verify sample preparation and protein loading. Consider increasing stringency of wash buffers. Confirm specificity using a knockout cell line or siRNA knockdown as a negative control.
• Unexpected band size: Post-translational modifications (phosphorylation, glycosylation) may alter apparent molecular weight. Consult the literature for reported STK40 molecular weight variants.

For application-specific troubleshooting, contact Aladdin Scientific technical support with your experimental details.

Ordering, Shipping, and Returns

STK40 Mouse mAb is available from Aladdin Scientific under catalog number Ab349965. To place an order, visit the Aladdin Scientific website, search by SKU or product name, and add to cart. For bulk or custom orders, contact our sales team directly.

Shipping: Antibody products are typically shipped on dry ice or with cold packs to maintain the cold chain. Please inspect the package upon arrival and contact us immediately if the cold chain appears to have been compromised.

Returns and Refunds: If the product does not perform as described in the validated applications listed on the datasheet, please contact Aladdin Scientific customer service within the eligible return window with your order details, batch number, and a description of the issue. Our team will work with you to resolve the concern, which may include replacement or refund per our returns policy.

Frequently asked questions

What is the catalog number for STK40 Mouse mAb from Aladdin Scientific?

The catalog number for STK40 Mouse mAb at Aladdin Scientific is Ab349965. You can search this SKU directly on the Aladdin Scientific website to access the product page and datasheet.

In which species was the STK40 Mouse mAb raised, and why does this matter?

STK40 Mouse mAb was raised in mouse (Mus musculus). This is important because when selecting a secondary antibody, you must choose one that is directed against mouse immunoglobulins (e.g., anti-mouse IgG). Additionally, if you are working with mouse-derived samples, be aware of potential non-specific signals from endogenous immunoglobulins; consider using a mouse-on-mouse detection kit if needed.

How should I store STK40 Mouse mAb to preserve its activity?

As a general guideline for monoclonal antibodies, STK40 Mouse mAb should be stored at –20 °C for long-term preservation, and at 4 °C for short-term use. Repeated freeze-thaw cycles should be avoided; it is recommended to aliquot the antibody after the first thaw. Always refer to the specific storage conditions stated on the Ab349965 product datasheet.

What applications is STK40 Mouse mAb validated for?

Specific validated applications for Ab349965 are listed on the official product datasheet. Common applications for mouse monoclonal antibodies targeting intracellular kinases include Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), and Immunoprecipitation (IP). Please consult the Ab349965 datasheet for confirmed application details and recommended dilutions.

What should I do if I experience no signal when using STK40 Mouse mAb in Western Blot?

If you observe no signal, first verify that STK40 is expressed in your sample by checking published expression data or using a positive control lysate. Next, confirm that your secondary antibody recognizes mouse IgG and that your dilutions are within the recommended range. Also check that antigen is not masked by over-fixation or inappropriate sample preparation conditions. Contact Aladdin Scientific technical support if issues persist.

Can I use STK40 Mouse mAb for flow cytometry?

Flow cytometry compatibility for Ab349965 should be confirmed on the official product datasheet, as not all antibodies are validated for every application. If flow cytometry is not listed as a validated application, we recommend contacting Aladdin Scientific technical support to discuss feasibility and any available data.

References

1. Aladdin Scientific product page for STK40 Mouse mAb, catalog number Ab349965. Reference OEM code: absea#KC-36962.
2. General principles of monoclonal antibody production, storage, and application: textbook-level guidance applicable to mouse-derived monoclonal antibodies targeting serine/threonine kinases.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.