DNA methylation is an important epigenetic regulatory mechanism in mammals, among which 5-methylcytosine (5mC) is the major form studied. 5mC is established and maintained by the DNMT family, and after sequential oxidation by the TET family, it can be removed through replication-dependent ...
Topic
Epigenetics
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
4 resources · protocols, FAQs & articles
Most somatic cells in the human body contain essentially the same deoxyribonucleic acid (DNA) sequence, yet different cells differ in morphology, function, and gene expression patterns. Hepatocytes express metabolism-related genes; neurons express genes involved in synaptic transmission; ...
MAT catalyzes the conversion of methionine and ATP into S-adenosylmethionine (SAM). SAM is not only one of the most central active methyl donors in cells, but also lies at the intersection of one-carbon metabolism, epigenetic regulation, and stress adaptation. As mechanisms involving MAT1A/MAT2A ...
Epigenetics refers to modifications in gene activity that occur without altering the underlying DNA sequence. Rather than changing the genetic code, these processes regulate whether genes are switched on or off through mechanisms such as chromatin remodeling. This often involves chemical changes ...
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Techniques often explored alongside immunological experiments.