Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated pour les flux de travail chromatographiques et analytiques sensibles nécessitant une interférence de ligne de base minimale.

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Stockage & expédition

Store at -20°C,Avoid repeated freezing and thawing Expédition Ice chest + Ice pads. Consultez le COA spécifique au lot pour les spécifications exactes.

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Documents qualité

SDS, COA, fiche technique et fiche de spécifications disponibles au téléchargement. COA spécifique au lot accessible via la recherche par numéro de lot.

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Preuve documentaire

Cité dans 0 publications à comité de lecture couvrant la chromatographie, la synthèse organique et les réactions de couplage croisé.

Vue d’ensemble

Mouse anti Human HDAC1 Antibody, Monoclonal (E22), could be used for WB, IHC, IF, ICC and so on.

Application:

WB: 0.2-2μg/mL

IHC: 5-20μg/mL

ICC/IF: 5-20μg/mL

Protein Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

Post-translational

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.

Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.

Ubiquitinated by CHFR, leading to its degradation by the proteasome.

Spécifications

Product Name

HDAC1 Mouse mAb - Anticorps primaire, specific to HDAC1, Mouse IgG1

Synonymes

Spécifications et pureté

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Espèce hôte

Souris

Spécificité

HDAC1

Immunogène

Recombinant HDAC1 expressed in E.coli (AA 1-482) .

Contrôle positif

WB: A549, HeLa, HepG2, Jurkat, THP-1, HEK293 and NIH/3T3 cell lysates; IHC: Human Placenta Tissue, Human Lung cancer Tissue, Rat Colon Tissue, Rat Testis Tissue, Mouse Pancreas Tissue, Mouse Cerebellum Tissue; IF: HeLa.

Conjugation

Unconjugated

Grade

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotype

Mouse IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A+G Affinity Chromatography

Concentration

See COA

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: HDAC1 Mouse mAb (Ab107009)

HDAC1 Mouse mAb (Ab107009) - Western Blot
All lanes: HDAC1 Mouse mAb (Ab107009) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 63 kDa
Exposure time: 1.2 s