HDAC4 Mouse mAb - Anticorps primaire, specific to HDAC4, Mouse IgG2a

Cat. No.: Ab107057

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IP, WB

Host species

Souris

Reactive species

conseil, Humain, Monkey, Souris

Isotype

Mouse IgG2a

Clone Number

4A3-H7-C6

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human HDAC4 Antibody, Monoclonal antibody(4A3-H7-C6), could be used for WB, IP and so on.
Application:
WB: 1/500-1/1000
IP: 1/20
Protein Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation via its interaction with the myocyte enhancer factors such as MEF2A, MEF2C and MEF2D.
Post-translational
Phosphorylated by CaMK4 at Ser-246, Ser-467 and Ser-632. Phosphorylation at other residues is required for the interaction with 14-3-3. Sumoylation on Lys-559 is promoted by the E3 SUMO-protein ligase RANBP2, and prevented by phosphorylation by CaMK4.

Specifications

Product Name

HDAC4 Mouse mAb - Anticorps primaire, specific to HDAC4, Mouse IgG2a

Synonymes

AHO3 antibody | BDMR antibody | EC 3.5.1.98 antibody | HA6116 antibody | HD 4 antibody | HD4 antibody | HDAC 4 antibody | HDAC A antibody | HDAC4 antibody | HDAC4_HUMAN antibody | HDACA antibody | Histone deacetylase 4 antibody | Histone Deacetylase A ant

Spécifications et pureté

ExactAb™, Validated, 1.0 mg/mL

Espèce hôte

Souris

Spécificité

HDAC4

Immunogène

Recombinant protein of human HDAC4 (AA 11-125)

Contrôle positif

WB: Jurkat, NIH/3T3 and C6 cell lysates. IP: HeLa lysates.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

Isotype

Mouse IgG2a

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein G purified

Concentration

1.0 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: HDAC4 Mouse mAb (Ab107057)

HDAC4 Mouse mAb (Ab107057) - Western Blot
All lanes: HDAC4 Mouse mAb (Ab107057) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 119 kDa
Observed band size: 134 kDa
Exposure time: 60.0 s

Western Blot: HDAC4 Mouse mAb (Ab107057)

HDAC4 Mouse mAb (Ab107057) - Western Blot
All lanes: HDAC4 Mouse mAb (Ab107057) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 119 kDa
Observed band size: 134 kDa
Exposure time: 30.0 s

Western Blot: HDAC4 Mouse mAb (Ab107057)

HDAC4 Mouse mAb (Ab107057) - Western Blot
Immunoprecipitation analysis of HDAC4 in HeLa lysates using HDAC4 Mouse mAb (Ab107057)

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IP:

  1/20;

Cibles associées (humaines)

HDAC4 Tclin Histone deacetylase 4 (0 Activities)

Discover Target: HDAC4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HDAC4 Tclin Histone deacetylase 4 (2328 Activities)

Discover Target: HDAC4

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

HDAC4 Tclin Cereblon/Histone deacetylase 4 (8 Activities)

Discover Target: HDAC4

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action

Références

1. Yong Ling, Yangyang Li, Rui Zhu, Jianqiang Qian, Ji Liu, Weijie Gao, Chi Meng, Jiefei Miao, Biao Xiong, Xiaodong Qiu, Changchun Ling, Hong Dai, Yanan Zhang.  (2019)  Hydroxamic Acid Derivatives of β-Carboline/Hydroxycinnamic Acid Hybrids Inducing Apoptosis and Autophagy through the PI3K/Akt/mTOR Pathways.  JOURNAL OF NATURAL PRODUCTS,      [PMID:31120744] [10.1021/acs.jnatprod.8b00843]

Foire aux questions

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HDAC4 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.