KPNA2 Mouse mAb - Anticorps primaire, specific to KPNA2, Mouse IgG1

Cat. No.: Ab112361

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, See COA

Application

IHC, WB

Host species

Souris

Reactive species

conseil, Humain, Pig, Souris

Isotype

Mouse IgG1

Clone Number

C8

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human KPNA2 Antibody, Monoclonal (C8), could be used for WB, IHC and so on.
Application:
WB: 0.01-3µg/mL
IHC : 5-20µg/mL
Protein Function
Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.

Specifications

Product Name

KPNA2 Mouse mAb - Anticorps primaire, specific to KPNA2, Mouse IgG1

Synonymes

Anti-KPNA2 antibody | IMA1_HUMAN antibody | Importin alpha 1 antibody | Importin alpha 2 antibody | Importin alpha 2 subunit antibody | Importin alpha P1 antibody | Importin subunit alpha-1 antibody | IPO A1 antibody | IPOA 1 antibody | IPOA1 antibody | K

Spécifications et pureté

Carrier Free, ExactAb™, Validated, High Performance, See COA

Espèce hôte

Souris

Spécificité

KPNA2

Immunogène

Recombinant KPNa2 expressed in E.coli (AA 157-412).

Contrôle positif

WB: Jurkat, HEK293, HeLa, Hep G2, A549, NIH/3T3 and C6 cell lysates. IHC: Human kidney tissue.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, High Performance, Validated

Isotype

Mouse IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A + Protein G purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: KPNA2 Mouse mAb (Ab112361)

[Ab112361] KPNA2 Mouse mAb - Western Blot
All lanes: KPNA2 Mouse mAb (Ab112361) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 58 kDa
Observed band size: 63 kDa
Exposure time: 6.6 s

IHC: KPNA2 Mouse mAb (Ab112361)

KPNA2 Mouse mAb (Ab112361) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 10 µg/mL KPNA2 Mouse mAb (Ab112361)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Application

• Application: Dilution info
• WB:

  0.01-3 µg/mL;

• IHC:

  5-20 µg/mL;

Cibles associées (humaines)

KPNA2 Tbio Importin subunit alpha-1 (0 Activities)

Discover Target: KPNA2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (KPNA2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.