LRRN1 Mouse mAb - Anticorps primaire, specific to LRRN1

Cat. No.: Ab113634

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IP, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG1

Clone Number

K16288_6D3

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human LRRN1 Antibody, Monoclonal (K16288_6D3), could be used for WB, IP and so on.

Specifications

Product Name

LRRN1 Mouse mAb - Anticorps primaire, specific to LRRN1

Synonymes

NLRR1 | NLRR-1 | FIGLER3 | fibronectin type III, immunoglobulin and leucine rich repeat domains 3 | KIAA1497 | leucine rich repeat neuronal 1 | leucine-rich repeat neuronal protein 1 | LRN | LRRN1 | Neuronal leucine-rich repeat protein 1 | FIGLER3 antibod

Spécifications et pureté

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Espèce hôte

Souris

Spécificité

LRRN1

Immunogène

Recombinant protein of human LRRN1 (AA 31-630)

Contrôle positif

WB: SH-SY5Y cell lysate. IP: SH-SY5Y lysates.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -80°C,Avoid repeated freezing and thawing

Expédié en

Dry ice packs + Cold packs

Stabilité et stockage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: LRRN1 Mouse mAb (Ab113634)

LRRN1 Mouse mAb (Ab113634) - Western Blot
All lanes: LRRN1 Mouse mAb (Ab113634) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 81 kDa
Observed band size: 66 kDa
Exposure time: 100.0 s

IP: LRRN1 Mouse mAb (Ab113634)

LRRN1 Mouse mAb (Ab113634) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of LRRN1 Mouse mAb (Ab113634) with SH-SY5Y lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. LRRN1 Mouse mAb (Ab113634) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody.
Lane 1: SH-SY5Y lysate
Lane 2: LRRN1 immunoprecipitated from SH-SY5Y lysate by LRRN1 Mouse mAb (Ab113634)
Lane 3: The isotype control antibody instead of LRRN1 Mouse mAb (Ab113634) in HeLa lysate.Result: LRRN1 Mouse mAb (Ab113634) can immunoprecipitate LRRN1.

Application

• Application: Dilution info
• WB:

  1/1000 ;

• IP:

  1/100;

Cibles associées (humaines)

LRRN1 Tbio Leucine-rich repeat neuronal protein 1 (0 Activities)

Discover Target: LRRN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (LRRN1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.