MAPK11 Antibody - Anticorps primaire, specific to MAPK11, Rabbit IgG

Cat. No.: Ab119724

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.6 mg/mL

Application

IF/ICC, IHC, WB

Host species

Lapin

Reactive species

Humain

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human MAPK11 Antibody, Polyclonal antibody, could be used for WB, IHC and so on.
Application
WB: 1/500 - 1/1000
IHC: 1/50 - 1/100
Protein Function
Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.
Post-translational
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.

Specifications

Product Name

MAPK11 Antibody - Anticorps primaire, specific to MAPK11, Rabbit IgG

Synonymes

Human p38Beta MAP kinase mRNA complete cds antibody | MAP kinase 11 antibody | MAP kinase p38 beta antibody | MAPK 11 antibody | Mapk11 antibody | Mitogen activated protein kinase 11 antibody | Mitogen activated protein kinase p38 2 antibody | Mitogen act

Spécifications et pureté

ExactAb™, Validated, Carrier Free, 0.6 mg/mL

Espèce hôte

Lapin

Spécificité

MAPK11

Immunogène

A synthetic peptide derived from human MAPK11. (AA 251-364).

Contrôle positif

WB: HeLa, MCF7 and Jurkat cell lysates. IHC: Human brain tissue. IF/ICC: HeLa cells

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Rabbit IgG

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

0.6 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: MAPK11 Antibody (Ab119724)

MAPK11 Antibody (Ab119724) - Western Blot
All lanes: MAPK11 Antibody (Ab119724) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution

Predicted band size: 41 kDa
Observed band size: 38 kDa
Exposure time: 60.0 s

Western Blot: MAPK11 Antibody (Ab119724)

MAPK11 Antibody (Ab119724) - Western Blot
All lanes: MAPK11 Antibody (Ab119724) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 42 kDa
Observed band size: 39 kDa
Exposure time: 100.0 s

IHC: MAPK11 Antibody (Ab119724)

MAPK11 Antibody (Ab119724) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using MAPK11 Antibody (Ab119724). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: MAPK11 Antibody (Ab119724)

MAPK11 Antibody (Ab119724) - IF/ICC

IF analysis of MAPK11 Antibody (Ab119724) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MAPK11 Antibody (Ab119724) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

• Application: Dilution info
• WB:

  1/500-1/1000

• IHC:

  1/50 - 1/100;

• IF/ICC:

  1/200;

Cibles associées (humaines)

MAPK11 Tchem Mitogen-activated protein kinase 11 (0 Activities)

Discover Target: MAPK11

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MAPK11 Tchem MAP kinase p38 beta (2785 Activities)

Discover Target: MAPK11

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action

Références

1. Peng Zhu, Jianqiang Qian, Zhongyuan Xu, Chi Meng, Ji Liu, Wenpei Shan, Weizhong Zhu, Yongjun Wang, Yumin Yang, Wei Zhang, Yanan Zhang, Yong Ling.  (2020)  Piperlonguminine and Piperine Analogues as TrxR Inhibitors that Promote ROS and Autophagy and Regulate p38 and Akt/mTOR Signaling.  JOURNAL OF NATURAL PRODUCTS,      [PMID:33026807] [10.1021/acs.jnatprod.0c00599]

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MAPK11 Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.