QUALITÉ & PURETÉCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.1.0 mg/mL
ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated pour les flux de travail chromatographiques et analytiques sensibles nécessitant une interférence de ligne de base minimale.
🌡
Stockage & expédition
Store at -80°C,Avoid repeated freezing and thawing Expédition Dry ice packs + Cold packs. Consultez le COA spécifique au lot pour les spécifications exactes.
📋
Documents qualité
SDS, COA, fiche technique et fiche de spécifications disponibles au téléchargement. COA spécifique au lot accessible via la recherche par numéro de lot.
📚
Preuve documentaire
Cité dans 0 publications à comité de lecture couvrant la chromatographie, la synthèse organique et les réactions de couplage croisé.
Vue d’ensemble
Mouse anti Human MelanA Antibody, Monoclonal (KAA343_6B7), could be used for WB, IHC, IP and so on.
Application
WB: 1/1000
IHC: 1/200
IP: 1/100
Protein Function
Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Spécifications
Product Name
MelanA Mouse mAb - Anticorps primaire, specific to MelanA, Mouse IgG1
Synonymes
Antigen LB39 AA antibody | Antigen LB39-AA antibody | Antigen SK29 AA antibody | Antigen SK29-AA antibody | MAR1_HUMAN antibody | MART 1 antibody | MART-1 antibody | MART1 antibody | Melan A antibody | Melan A protein antibody | Melanoma antigen recognize
Store at -80°C,Avoid repeated freezing and thawing
Expédié en
Dry ice packs + Cold packs
Stabilité et stockage
Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
MelanA Mouse Antibody (Ab114904) - Western Blot 15 µg of SK-MEL-28 lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. MelanA Mouse Antibody (Ab114904) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. MLANA band was visualized using ECL Western Blotting Substrate. Result: MelanA Mouse Antibody (Ab114904) can detect MLANA by Western blotting.
MelanA Mouse Antibody (Ab114904) - IP Immunoprecipitation was performed by incubation of 2.5 µg of MelanA Mouse Antibody (Ab114904) with SK-MEL-28 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. MelanA Mouse Antibody (Ab114904) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody. Lane 1: SK-MEL-28 lysate Lane 2: MLANA immunoprecipitated from SK-MEL-28 lysate by MelanA Mouse Antibody (Ab114904) Lane 3: The same as Lane 2 was IgG isotype control antibody Result: MelanA Mouse Antibody (Ab114904) can immunoprecipitate MLANA.
MelanA Mouse Antibody (Ab114904) - IHC IHC-P analysis of malignant melanoma tissue by MelanA Mouse Antibody (Ab114904).IHC-P was performed using sections of the formalin-fixed paraffin-embedded malignant melanoma tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with MelanA Mouse Antibody (Ab114904) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Tumor cells are positively stained at the cytoplasm.
Application
Application
Dilution info
WB
1/1000
IP
1/100
IHC
1/200
Documentation
📋 Fiche de données de sécurité (FDS)
Document complet couvrant les dangers, la manipulation, le stockage et la conformité réglementaire.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Avis
Avis des clients
Foire aux questions
How is this product shipped?
This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Carrier Free mean?
Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.
How should this product be stored?
Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MelanA Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Besoin d'aide pour choisir la qualité ?
Notre guide de sélection des qualités couvre la pureté, le statut du stabilisant et l'adéquation à l'application pour toutes les variantes de notre catalogue.
Nous utilisons des cookies pour garantir le bon fonctionnement du site et, lorsque cela est autorisé, pour améliorer votre expérience. Vous pouvez gérer vos préférences à tout moment dans les Paramètres. Pour en savoir plus, consultez notre Politique relative aux cookies.
Souhaitez-vous que nous vous envoyions un message lorsque des réductions seront disponibles?
Me rappeler plus tard
Merci ! Veuillez vérifier votre boîte de réception e-mail pour confirmer.
Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
