QUALITÉ & PURETÉBioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.Ready-to-use?Ready-to-use — supplied pre-formulated at working concentration, no prep needed. Use to save time and reduce pipetting/dilution errors.Suitable for Immunohistochemistry(IHC)?IHC grade — validated for immunohistochemistry on tissue sections. Use to detect and localize antigens in fixed tissue.
BioReagent, ready-to-use, Suitable for Immunohistochemistry BioReagent,Prêt à l'emploi,Suitable for Immunohistochemistry(IHC) pour les flux de travail chromatographiques et analytiques sensibles nécessitant une interférence de ligne de base minimale.
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Stockage & expédition
Protected from light,Room temperature Expédition Normal. Consultez le COA spécifique au lot pour les spécifications exactes.
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Documents qualité
SDS, COA, fiche technique et fiche de spécifications disponibles au téléchargement. COA spécifique au lot accessible via la recherche par numéro de lot.
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Preuve documentaire
Cité dans 0 publications à comité de lecture couvrant la chromatographie, la synthèse organique et les réactions de couplage croisé.
Vue d’ensemble
Methylgreen-pyronine is a common dye used in tissue or cell staining to color the nucleus green or blue-green and the nucleoli in the cytoplasm and nucleus red. Methyl green can bind to DNA in the nucleus, thus producing nuclear staining; Pyronin can bind to RNA in the cytoplasm or nucleolus, so that the cytoplasm and nucleolus can be stained. Operation process: 1 Sample handling 1.1 For paraffin sections: dewaxing in xylene for 2×5min; Anhydrous ethanol 5 min, 90% ethanol 2 min, 70% ethanol 2 min; Rinse with PBS for 2 min. 1.2 For frozen sections: Rinse with PBS for 2 min. 1.3 For cultured cells: fixed with 4% paraformaldehyde for more than 10 min and washed with PBS for 2×2 min. 2 methyl green-Pyronine staining for the above treated samples: 2.1 Methyl green-Pyronine dyeing solution staining for about 5 ~10min (the time can be adjusted according to the dyeing results and requirements). 2.2 Wash distilled water 2-3 times (each time for seconds). 3 Dehydration, transparency, sealing or other dyeing 3.1 Dehydration of acetone 3 times, 1~2sec(3 dips) each time. 3.2 Wash with acetone/xylene (1:1) twice, 1~2min each time. Xylene transparent 2×1~2min. 3.3 Seal the tablet with neutral gum or other sealant. 3.4 Microscopically, the nucleus appears green or blue-green, while the cytoplasm and nucleolus appear red. Staining results: the nucleus is dyed green or blue-green, and the nucleoli in the cytoplasm and nucleus are dyed red. Note: 1 Need to bring 4% polyformaldehyde, gradient ethanol, xylene, neutral gum or other tablets. 2 When using this kit for the first time, it is recommended to take 1 to 2 samples for pre-test. Warm reminder: For your own safety, please take good protection before using the reagent, such as wearing a lab coat, gloves, etc.
Spécifications
Synonymes
Methyl Green-Pyronin Staining Solution
Spécifications et pureté
BioReagent, ready-to-use, Suitable for Immunohistochemistry
Stabilité et stockage
Store at Room temperature long term (12 months). Upon receipt, it is recommended to aliquot. Store in the dark.
Conditions de stockage de stockage
Protected from light,Room temperature
Expédié en
Normal
Grade
BioReagent, Prêt à l'emploi, Suitable for Immunohistochemistry(IHC)
Product Line
Produits biologiques
Documentation
📋 Fiche de données de sécurité (FDS)
Document complet couvrant les dangers, la manipulation, le stockage et la conformité réglementaire.
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