MMP2 Mouse mAb - Anticorps primaire, specific to MMP2, Mouse IgG1

Cat. No.: Ab115657

GRADE & PURITY Carrier Free, Validated, ExactAb™, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Souris

Reactive species

conseil, Humain, Souris

Isotype

Mouse IgG1

Clone Number

C1

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human MMP2 Antibody, Monoclonal (C1), could be used for WB, IHC and so on.

Specifications

Product Name

MMP2 Mouse mAb - Anticorps primaire, specific to MMP2, Mouse IgG1

Synonymes

72 kDa gelatinase | CLG4 | CLG4A72 kDa type IV collagenase | collagenase type IV-A | EC 3.4.24 | EC 3.4.24.24 | Gelatinase A | matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IVcollagenase) | matrix metalloproteinase 2 (gelatinase A,

Spécifications et pureté

Carrier Free, ExactAb™, Validated, High Performance, See COA

Mécanismes biochimiques et physiologiques

Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins,

Espèce hôte

Souris

Spécificité

MMP2

Immunogène

Recombinant MMP2 expressed in E.coli (AA 110-660)

Contrôle positif

WB: RAW 264.7, C6, HUVEC, A431, SK-N-SH and MCF7 cell lysates. IHC: Human kidney tissue. IF/ICC: HeLa cells.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, High Performance, Validated

Isotype

Mouse IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A + Protein G purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: MMP2 Mouse mAb (Ab115657)

MMP2 Mouse mAb (Ab115657) - Western Blot
All lanes: MMP2 Mouse mAb (Ab115657) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution

Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 29.4 s

IHC: MMP2 Mouse mAb (Ab115657)

MMP2 Mouse mAb (Ab115657) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 20 µg/mL MMP2 Mouse mAb (Ab115657)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IF/ICC: MMP2 Mouse mAb (Ab115657)

MMP2 Mouse mAb (Ab115657) - IF/ICC
IF analysis of MMP2 Mouse mAb (Ab115657) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with MMP2 Mouse mAb (Ab115657) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Application

• Application: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.01-2 µg/mL ;

• IF/ICC:

  5 µg/mL;

Cibles associées (humaines)

MMP2 Tchem 72 kDa type IV collagenase (0 Activities)

Discover Target: MMP2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MMP2 Tchem Matrix metalloproteinase-2 (6627 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem Collagenase (153 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem Matrix metalloproteinase 2/9 (2 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem MMP-2/MMP-13 (113 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

MMP2 Tchem MMP-2/MMP-9 (120 Activities)

Discover Target: MMP2

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MMP2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.