MTHFD2 Mouse mAb - Anticorps primaire, specific to MTHFD2, Mouse IgG1

Cat. No.: Ab116276

QUALITÉ & PURETÉ Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG1

Clone Number

K70015_2H12

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human MTHFD2 Antibody, Monoclonal (K70015_2H12), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/200
IP:1/100

Spécifications

Product Name

MTHFD2 Mouse mAb - Anticorps primaire, specific to MTHFD2, Mouse IgG1

Synonymes

NMDMC | MTHFD2 | Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody | Descriptions antibody | Methenyltetrahydrofolate cyclohydrolase antibody | methylene tetrahydrofolate dehydrogenase (NAD+ dependent) antibody |

Spécifications et pureté

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Espèce hôte

Souris

Spécificité

MTHFD2

Immunogène

Recombinant protein of human MTHFD2 (AA 101-300)

Contrôle positif

WB: A431, Hep G2, HeLa, HEK293 and MOLT-4 cell lysates. IHC: Human kidney tissue. IP: A431 lysates.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -80°C,Avoid repeated freezing and thawing

Expédié en

Dry ice packs + Cold packs

Stabilité et stockage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: MTHFD2 Mouse mAb (Ab116276)

MTHFD2 Mouse mAb (Ab116276) - Western Blot
All lanes: MTHFD2 Mouse mAb (Ab116276) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 34 kDa
Exposure time: 169.7 s

IP: MTHFD2 Mouse mAb (Ab116276)

MTHFD2 Mouse mAb (Ab116276) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of MTHFD2 Mouse mAb (Ab116276) with A431 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. MTHFD2Mouse mAb (Ab116276) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: A431 lysateLane 2: MTHFD2 immunoprecipitated from A431 lysate by MTHFD2 Mouse mAb (Ab116276) 
Lane 3: IgG isotype control antibody instead of MTHFD2 Mouse mAb (Ab116276) in A431 lysate.
Result: MTHFD2 Mouse mAb (Ab116276) can immunoprecipitate MTHFD2.

IHC: MTHFD2 Mouse mAb (Ab116276)

MTHFD2 Mouse mAb (Ab116276) - IHC
IHC-P analysis of kidney tissue by MTHFD2 Mouse mAb (Ab116276).IHC-P was performed using sections of the formalin-fixed paraffin-embedded kidney tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with MTHFD2 Mouse mAb (Ab116276) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in tubules are positively stained at the cytoplasm.

Application

Application

Dilution info

WB

1/1000

IP

1/100

IHC

1/200

Cibles associées (humaines)

MTHFD2 Tchem Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial (0 Activities)

Discover Target: MTHFD2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MTHFD2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.