NUCB2 Mouse mAb - Anticorps primaire, specific to NUCB2

Cat. No.: Ab118866

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG1

Clone Number

K70044_2H5

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human NUCB2 Antibody, Monoclonal (K70044_2H5), could be used for WB, IHC, IP and so on.

Specifications

Product Name

NUCB2 Mouse mAb - Anticorps primaire, specific to NUCB2

Synonymes

DNA binding protein NEFA antibody | DNA-binding protein NEFA antibody | Gastric cancer antigen Zg4 antibody | HEL S 109 antibody | NEFA antibody | Nefastin 1 antibody | Nefastin-1 antibody | Nefastin1 antibody | Nucb2 antibody | NUCB2_HUMAN antibody | Nuc

Spécifications et pureté

ExactAb™, Carrier Free, Validated, 1.0 mg/mL

Mécanismes biochimiques et physiologiques

Calcium-binding protein. May have a role in calcium homeostasis.

Espèce hôte

Souris

Spécificité

NUCB2

Immunogène

Recombinant protein of human NUCB2 (AA 31-410)

Contrôle positif

WB: HL-60 lysates. IHC: Human stomach tissue. IP: HL-60 lysates.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -80°C,Avoid repeated freezing and thawing

Expédié en

Dry ice packs + Cold packs

Stabilité et stockage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: NUCB2 Mouse mAb(Ab118866)

NUCB2 Mouse mAb(Ab118866) - Western Blot
All lanes: NUCB2 Mouse mAb(Ab118866) at 1/500 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 100.0 s

IHC: NUCB2 Mouse mAb(Ab118866)

NUCB2 Mouse mAb(Ab118866) - IHC
IHC-P analysis of stomach tissue by NUCB2 Mouse mAb(Ab118866).
IHC-P was performed using sections of the formalin-fixed paraffin-embedded stomach tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with NUCB2 Mouse mAb(Ab118866) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glandular cells are positively stained at the cytoplasm.

IP: NUCB2 Mouse mAb(Ab118866)

NUCB2 Mouse mAb(Ab118866) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of NUCB2 Mouse mAb(Ab118866) with HL-60 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. NUCB2 Mouse mAb(Ab118866) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Light chain specific) was used as the secondary antibody. The isotype control antibody was mouse IgG1.
Lane 1: HL-60 lysate
Lane 2: NUCB2 immunoprecipitated from HL-60 lysate by NUCB2 Mouse mAb(Ab118866)
Lane 3: The same as Lane 2 was used as IgG isotype control antibody
Result: NUCB2 Mouse mAb(Ab118866) can immunoprecipitate NUCB2.

Application

• Application: Dilution info
• IP:

  1/100;

• IHC:

  1/200 ;

• WB:

  1/500;

Cibles associées (humaines)

NUCB2 Tbio Nucleobindin-2 (0 Activities)

Discover Target: NUCB2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (NUCB2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.