PCMT1 Mouse mAb - Anticorps primaire, specific to PCMT1

Cat. No.: Ab120677

GRADE & PURITY Validated, ExactAb™, See COA

Application

IHC, WB

Host species

Souris

Reactive species

conseil, Humain, Souris

Isotype

Mouse IgG2b

Clone Number

OTI2A9

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human PCMT1 Antibody, Monoclonal (OTI2A9), could be used for WB, IHC and so on.

Specifications

Product Name

PCMT1 Mouse mAb - Anticorps primaire, specific to PCMT1

Synonymes

L isoaspartyl protein carboxyl methyltransferase antibody | L isoaspartyl/D aspartyl methyltransferase antibody | L-isoaspartyl protein carboxyl methyltransferase antibody | PCMT 1 antibody | Pcmt1 antibody | PIMT antibody | PIMT_HUMAN antibody | Protein

Spécifications et pureté

ExactAb™, Validated, See COA

Mécanismes biochimiques et physiologiques

Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins. Acts on E

Espèce hôte

Souris

Spécificité

PCMT1

Immunogène

Recombinant protein of human PCMT1 (AA 60-285).

Contrôle positif

WB: HeLa, Jurkat, MCF7, K562, NIH/3T3 and C6 cell lysates. IHC: Human breast, colon, kidney, lung, pancreas tissues.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: PCMT1 Mouse mAb (Ab120677)

PCMT1 Mouse mAb (Ab120677) - Western Blot
All lanes: PCMT1 Mouse mAb (Ab120677) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 3.5 s

IHC: PCMT1 Mouse mAb (Ab120677)

PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human breast tissue tissue using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).

IHC: PCMT1 Mouse mAb (Ab120677)

PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human colon tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).

IHC: PCMT1 Mouse mAb (Ab120677)

PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human Kidney tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, Ab120677) (1/2000).

IHC: PCMT1 Mouse mAb (Ab120677)

Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human lung tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).

IHC: PCMT1 Mouse mAb (Ab120677)

PCMT1 Mouse mAb (Ab120677) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using PCMT1 Mouse mAb (Ab120677). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 min, Ab120677) (1/2000).

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IHC:

  1/2000;

Cibles associées (humaines)

PCMT1 Tbio Protein-L-isoaspartate(D-aspartate) O-methyltransferase (0 Activities)

Discover Target: PCMT1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PCMT1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.