PGAM2 Mouse mAb - Anticorps primaire, specific to PGAM2

Cat. No.: Ab121443

GRADE & PURITY Validated, ExactAb™, See COA

Application

IF/ICC, WB

Host species

Souris

Reactive species

Cependant, conseil, Humain, Monkey, Souris

Isotype

Mouse IgG2a

Clone Number

OTI4B12

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human PGAM2 Antibody, Monoclonal (OTI4B12), could be used for WB, IF/ICC and so on.

Specifications

Product Name

PGAM2 Mouse mAb - Anticorps primaire, specific to PGAM2

Synonymes

BPG dependent PGAM 2 antibody | BPG-dependent PGAM 2 antibody | GSD10 antibody | MGC88743 antibody | Muscle specific phosphoglycerate mutase antibody | Muscle-specific phosphoglycerate mutase antibody | OTTHUMP00000207787 antibody | PGAM 2 antibody | PGAM

Spécifications et pureté

ExactAb™, Validated, See COA

Mécanismes biochimiques et physiologiques

Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase), but with a reduced activity.

Espèce hôte

Souris

Spécificité

PGAM2

Immunogène

Recombinant full length protein of human PGAM2.

Contrôle positif

WB: Hep G2, HeLa, Jurkat, MCF7, A431, NIH/3T3 and C6 cell lysates. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY PGAM2.

Conjugation

Unconjugated

Grade

ExactAb™, Validated

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

See COA

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: PGAM2 Mouse mAb (Ab121443)

PGAM2 Mouse mAb (Ab121443) - Western Blot
All lanes: PGAM2 Mouse mAb (Ab121443) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 29 kDa
Observed band size: 27 kDa
Exposure time: 3.1 s

IF/ICC: PGAM2 Mouse mAb (Ab121443)

PGAM2 Mouse mAb (Ab121443) - IF/ICC
PGAM2 Mouse mAb (Ab121443) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2.

Application

• Application: Dilution info
• WB:

  1/500-1/2000;

• IF/ICC:

  1/100;

Cibles associées (humaines)

PGAM2 Tbio Phosphoglycerate mutase 2 (0 Activities)

Discover Target: PGAM2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PGAM2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.