Phosphate acetyltransferase (PTA） , CAS No.9029-91-8

CAS: 9029-91-8 Cat. No.: P1431341 Numéro CE: 232-855-2

QUALITÉ & PURETÉ Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥3000 U/mL; ~3500 U/mg protein

Accession #

P39646

Bioactivity

≥3000 U/mL; ~3500 U/mg protein

Vue d’ensemble

High purity recombinant phosphotransacetylase (Bacillus subtilis) for use in research, biochemical enzyme assays and analytical testing applications. 

Specificity: 

Catalyses the reaction: Coenzyme A + acetyl phosphate = acetyl-CoA + phosphate

Temperature Optima: 25°C

pH Optima: 7.4

Application examples: Applications for the measurement of acetic acid in the food, fermentation, wine, beverage and dairy industries.

PROPERTIES 

1. ELECTROPHORETIC PURITY: 

- Single band on SDS-gel electrophoresis (MW ~ 36,953) 

- Single major band on isoelectric focusing (pI ~ 5.3) 

2. PHYSICOCHEMICAL PROPERTIES: Recommended conditions of use are at pH 7.4 and up to 25°C. 

3. STORAGE AND USE CONDITIONS/RECOMMENDATIONS: The enzyme is supplied as an ammonium sulphate suspension containing 0.5 mg/mL BSA and should be stored at 4°C. For assay, this enzyme should be diluted in 100 mM Tris.HCl buffer, pH 7.4 containing 1 mg/mL BSA. Swirl to mix the enzyme suspension immediately prior to use. 

Spécifications

Product Name

Phosphate acetyltransferase (PTA） , CAS No.9029-91-8

Synonymes

Acetyl-CoA:orthophosphate acetyltransferase | phosphate acetyltransferase | phosphoacylase | phosphotransacetylase | PTA

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Spécifications et pureté

Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥3000 U/mL; ~3500 U/mg protein

Bioactivité

≥3000 U/mL; ~3500 U/mg protein

Accession #

P39646

Poids moléculaire prévu

36,953

CAS

9029-91-8

Numéro de commission de l'enzyme

EC 2.3.1.8

Type de molécule

Enzyme

Product Line

Produits biologiques

Stockage et expédition

Concentration

≥3000 U/mL; ~3500 U/mg protein

Conditions de stockage de stockage

Store at 2-8°C

Expédié en

Wet ice

Stabilité et stockage

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot.

Définition de l'unité

One Unit of phosphotransacetylase activity is defined as the amount of enzyme required to release one µmole of acetyl-CoA per minute from Coenzyme A (0.44 mM) in Tris.HCl buffer (86 mM) pH 7.4 at 25°C.