RAB21 Mouse mAb - Anticorps primaire, specific to RAB21

Cat. No.: Ab124150

GRADE & PURITY Validated, ExactAb™, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Souris

Reactive species

conseil, Humain, Souris

Isotype

Mouse IgG2b

Clone Number

OTI4A6

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human RAB21 Antibody, Monoclonal (OTI4A6), could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

RAB21 Mouse mAb - Anticorps primaire, specific to RAB21

Spécifications et pureté

Validated, High Performance, ExactAb™, See COA

Espèce hôte

Souris

Spécificité

RAB21

Immunogène

Recombinant protein of human RAB21 (AA 1-225).

Contrôle positif

WB: Hep G2, HeLa, MCF7. Raji and NIH/3T3 cell lysates. IHC: Human pancreas tissue. IF/ICC: HeLa cells

Conjugation

Unconjugated

Grade

ExactAb™, High Performance, Validated

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

See COA

Note

Alternative Product Ab124148

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: RAB21 Mouse mAb (Ab124150)

RAB21 Mouse mAb (Ab124150) - Western Blot
All lanes: RAB21 Mouse mAb (Ab124150) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa
Exposure time: 18.2 s

IF/ICC: RAB21 Mouse mAb (Ab124150)

RAB21 Mouse mAb (Ab124150) - IF/ICC
Immunofluorescent analysis of HeLa cells, using RAB21 Mouse mAb (Ab124150).

IHC: RAB21 Mouse mAb (Ab124150)

RAB21 Mouse mAb (Ab124150) - IHC
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue within the normal limits using RAB21 Mouse mAb (Ab124150). Heat-induced epitope retrieval by EDTA solution buffer pH 8.0 at 120°C for 3 min.

Application

• Application: Dilution info
• WB:

  1/1000 ;

• IHC:

  1/150

• IF/ICC:

  1:100 ;

Cibles associées (humaines)

RAB21 Tbio Ras-related protein Rab-21 (0 Activities)

Discover Target: RAB21

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RAB21 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.