RALB Mouse mAb - Primary antibody, specific to RALB, Mouse IgG2a

Cat. No.: Ab124480

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Mouse

Reactive species

Human, Mouse, Rat

Isotype

Mouse IgG2a

Clone Number

K92021_15B6

Conjugation

Unconjugated

Overview

Mouse anti Human RALB Antibody, Monoclonal (K92021_15B6), could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/2000
Protein Function:
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles. Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells. Plays a role in the late stages of cytokinesis and is required for the abscission of the bridge joining the sister cells emerging from mitosis. Required for suppression of apoptosis.

Specifications

Product Name

RALB Mouse mAb - Primary antibody, specific to RALB, Mouse IgG2a

Synonyms

V ral simian leukemia viral oncogene homolog B antibody | GTP binding protein | RalB | RAS-like protein B | ras-related protein Ral-B | v-ral simian leukemia viral oncogene homolog B (ras related | GTP bindingprotein) | 5730472O18Rik antibody | dRalb anti

Specifications & Purity

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Host species

Mouse

Specificity

RALB

Immunogen

Recombinant human RALB (AA 31-200).

Positive Control

WB: Hep G2, HEK293, A431, HUVEC, NIH/3T3 and C6 cell lysates. IHC: Human testis tissue.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG2a

Light Chain Type

kappa

SDS-PAGE

150 kDa

Purification Method

Protein A purified

Concentration

1.0 mg/mL

Storage and Shipping

Shape

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Stability And Storage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: RALB Mouse mAb (Ab124480)

RALB Mouse mAb (Ab124480) - Western Blot
All lanes: RALB Mouse mAb (Ab124480) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 32.2 s

IHC: RALB Mouse mAb (Ab124480)

RALB Mouse mAb (Ab124480) - IHC
IHC analysis of human testis tissue by RALB Mouse mAb (Ab124480).
IHC was performed using sections of the formalin-fixed paraffin-embedded human testis tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with RALB Mouse mAb (Ab124480) at 0.5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Cells in seminiferous ducts are positively stained at the cytoplasm membrane.

Application

• Application: Dilution info
• WB:

  1/1000 ;

• IHC:

  1/2000;

Associated Targets(Human)

RALB Tbio Ras-related protein Ral-B (0 Activities)

Discover Target: RALB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mechanisms of Action