GRADE & PURITYRUO?Research Use Only — for laboratory research, not diagnostic or therapeutic use. Use in R&D; do not use in clinical diagnosis or patient treatment.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.Suitable for Flow Cytometry?Flow-cytometry grade — validated antibodies/reagents for FACS analysis. Use to stain and quantify cell populations by flow cytometry.0.5 mg/mL
RUO,Recombinant,ExactAb™,Azide Free,Suitable for Flow Cytometry,0.5 mg/mL
Mécanismes biochimiques et physiologiques
Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class I molecule:peptide com
Espèce hôte
conseil
Spécificité
Mouse CD8α
Immunogène
Mouse Spleen Cells or Thymocyte Membranes
Contrôle positif
Flow Cytometry: C57BL/6 mouse splenocytes
Conjugation
APC
Excitation(nm)
Red Laser(633nm)
Émission (nm)
660nm
Ex/Em(nm)
Ex:650nm;Em:660nm
Grade
Azide Free, ExactAb™, Recombinant, RUO, Suitable for Flow Cytometry
Type d'action
ANTIBODY
Type de chaîne lumineuse
kappa
Méthode de purification
Protein A purified
Concentration
0.5 mg/mL
Stockage et expédition
Conditions de stockage de stockage
Store at 2-8°C,Protected from light,Do not freeze
Expédié en
Wet ice,Do not freeze
Stabilité et stockage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Flow cytometry analysis of CD8α expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant CD8α Antibody (APC) (Ab302183) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (APC) (Ab180313).
Flow cytometry analysis of CD8α expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant CD8α Antibody (APC) (Ab302183) at 0.1μg/test for 1 hour at 4°C. Blue - Rat IgG (APC) (Ab180313). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤1.0 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Avis
Avis des clients
Foire aux questions
What does RUO mean?
RUO refers to material produced and controlled to meet the requirements of pharmaceutical manufacturing and regulated workflows. Depending on the specific designation (GMP, RUO, Bio-medical, Injection grade, etc.), this may include controlled manufacturing environments, validated processes, audit-ready documentation and traceability.
What does Recombinant mean?
Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.
How should this product be stored?
Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.
How is this product shipped?
This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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