Recombinant CHMP2B Antibody - Anticorps primaire, specific to CHMP2B, Rabbit IgG

Cat. No.: Ab096362

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.4 mg/mL

Application

WB

Host species

Lapin

Reactive species

conseil, Humain, Souris

Isotype

Rabbit IgG

Conjugation

Unconjugated

Vue d’ensemble

Rabbit anti Human CHMP2B Antibody, Recombinant, could be used for WB and so on.
Application
WB: 1/500-1/1000
Protein Function
Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.

Specifications

Product Name

Recombinant CHMP2B Antibody - Anticorps primaire, specific to CHMP2B, Rabbit IgG

Synonymes

ALS17 antibody | Charged multivesicular body protein 2b antibody | CHM2B_HUMAN antibody | CHMP family, member 2B antibody | CHMP2.5 antibody | CHMP2b antibody | Chromatin modifying protein 2b antibody | Chromatin-modifying protein 2b antibody | DMT1 antib

Spécifications et pureté

ExactAb™, Validated, Recombinant, 0.4 mg/mL

Espèce hôte

Lapin

Spécificité

CHMP2B

Immunogène

A synthetic peptide derived from human CHMP2B (AA 171-213)

Contrôle positif

WB: A431, HeLa, MCF7, A549, HEK293, NIH/3T3 and C6 cell lysates.

Conjugation

Unconjugated

Grade

ExactAb™, Recombinant, Validated

Isotype

Rabbit IgG

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

0.4 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -20°C,Avoid repeated freezing and thawing

Expédié en

Ice chest + Ice pads

Stabilité et stockage

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: Recombinant CHMP2B Antibody (Ab096362)

Recombinant CHMP2B Antibody (Ab096362) - Western Blot
All lanes: Recombinant CHMP2B Antibody (Ab096362) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 24 kDa
Observed band size: 30 kDa
Exposure time: 1.1 s

Application

Application

Dilution info

WB

1/500-1/1000

Cibles associées (humaines)

CHMP2B Tbio Charged multivesicular body protein 2b (0 Activities)

Discover Target: CHMP2B

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant CHMP2B Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.