GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
ExactAb™, Validated, High Performance, See COA ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Vue d’ensemble
Mouse anti Human RFC4 Antibody, Monoclonal (OTI1A8), could be used for WB, IHC, IF/ICC and so on.
Specifications
Product Name
RFC4 Mouse mAb - Anticorps primaire, specific to RFC4
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit may be involved in the elongation of the multiprimed DNA templ
Espèce hôte
Souris
Spécificité
RFC4
Immunogène
Recombinant full length protein of human RFC4 (AA 1-363).
Contrôle positif
WB: Hep G2, HeLa, A549, Jurkat, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human pancreas, pancreas carcinoma, tonsil tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY RFC4.
Conjugation
Unconjugated
Grade
ExactAb™, High Performance, Validated
SDS-PAGE
150 kDa
Méthode de purification
Protein G purified
Concentration
See COA
Stockage et expédition
Forme
Liquid
Conditions de stockage de stockage
Store at -20°C,Avoid repeated freezing and thawing
Expédié en
Ice chest + Ice pads
Stabilité et stockage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
RFC4 Mouse mAb (Ab125155) - Western Blot All lanes: RFC4 Mouse mAb (Ab125155) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 40 kDa Observed band size: 38 kDa Exposure time: 61.2 s
RFC4 Mouse mAb (Ab125155) - IHC Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155)
RFC4 Mouse mAb (Ab125155) - IHC Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155).
RFC4 Mouse mAb (Ab125155) - IHC Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using RFC4 Mouse mAb (Ab125155). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 120°C for 3 min, Ab125155).
RFC4 Mouse mAb (Ab125155) - IF/ICC RFC4 Mouse mAb (Ab125155) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RFC4.
Application
Application
Dilution info
IF/ICC
1/100
IHC
1/150
WB
1/200-1/4000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Determine the necessary mass, volume, or concentration for preparing a solution.
Dilution Calculator
Determine the dilution needed to prepare a stock solution.
Reconstitution Calculator
Avis
Avis des clients
Foire aux questions
What does ExactAb? mean?
ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
What does Validated mean?
Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.
How should this product be stored?
Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.
How is this product shipped?
This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.
What documentation is provided?
Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.
Which species reactivity has this antibody been validated against?
Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (RFC4 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.
Need help choosing the grade?
Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
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