TNF alpha Mouse mAb - Anticorps primaire, specific to Human TNF alpha

Cat. No.: Ab156547
DISPONIBLE À COMMANDE
QUALITÉ & PURETÉ Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. ≥95%(SDS-PAGE) 1.0 mg/mL
Application
Capture Antibody, ELISA
Host species
Souris
Reactive species
Humain
Isotype
Mouse IgG1
Clone Number
C11/10B2
Conjugation
Unconjugated
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Taille
Allemagne (EU)
USA*
Prix
Qté
10μg
Ab156547-10μg
—
En stock ≥10
65,86€
50μg
Ab156547-50μg
—
En stock ≥10
194,29€
100μg
Ab156547-100μg
—
4 En stock
312,30€
1mg
Ab156547-1mg
Sur commande · 8–12 semaines
888,48€
Enter a quantity for the sizes you want to add.
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Pourquoi cette qualité

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL Animal Free,Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated pour les flux de travail chromatographiques et analytiques sensibles nécessitant une interférence de ligne de base minimale.

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Stockage & expédition

Store at -20°C,Avoid repeated freezing and thawing Expédition Ice chest + Ice pads. Consultez le COA spécifique au lot pour les spécifications exactes.

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Documents qualité

SDS, COA, fiche technique et fiche de spécifications disponibles au téléchargement. COA spécifique au lot accessible via la recherche par numéro de lot.

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Preuve documentaire

Cité dans 0 publications à comité de lecture couvrant la chromatographie, la synthèse organique et les réactions de couplage croisé.

Vue d’ensemble

Mouse anti Human TNF alpha Antibody, clone C11/10B2, could be used for a Capture Antibody in sandwich ELISAs

Spécifications

Product Name
TNF alpha Mouse mAb - Anticorps primaire, specific to Human TNF alpha
Synonymes
APC1 antibody | APC1 protein antibody | Cachectin antibody | DIF antibody | Differentiation inducing factor antibody | Macrophage cytotoxic factor antibody | Tnf antibody | TNF superfamily member 2 antibody | TNF superfamily, member 2 antibody | TNF, macr
Spécifications et pureté
Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL
Mécanismes biochimiques et physiologiques
Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is i
Espèce hôte
Souris
Spécificité
Human TNF alpha
Immunogène
Recombinant protein corresponding to Human TNF alpha
Conjugation
Unconjugated
Grade
Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Type de chaîne lumineuse
kappa
SDS-PAGE
150 kDa
Méthode de purification
Protein A purified
Paires appariées
[Ab215301] TNF alpha Mouse mAb, Detection Antibody
Concentration
1.0 mg/mL
Product Line
Produits biologiques
Stockage et expédition
Conditions de stockage de stockage
Store at -20°C,Avoid repeated freezing and thawing
Expédié en
Ice chest + Ice pads
Stabilité et stockage
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Images

Sandwich ELISA - TNF alpha standard curve
Sandwich ELISA detection of Recombinant Human TNF-α Protein (rp156007) using TNF alpha Mouse mAb (Ab156547) as capture antibody at concentration of 1.0 µg/mL. Biotinylated TNF alpha Mouse mAb (Ab215301) as detection antibody at concentration of 0.5 µg/mL. Detection of the standard curve was achieved by incubating Streptavidin-HRP (np156148).
Detection range: 0.97 ng/mL - 1000.0 ng/mL

Indirect ELISA - TNF alpha standard curve
Immobilized Recombinant Human TNF-α Protein (rp156007) at 1.0 µg/mL  overnight at 4℃. Coated protein probed with TNF alpha Mouse mAb (Ab156547). Goat Anti-Mouse IgG H&L (HRP) (Ab156255) detected bound primary antibody.
EC50: 59.41 ng/mL

Application
Application
Dilution info
ELISA

1.0 µg/mL as a Capture antibody in ELISA

Documentation

📋 Fiche de données de sécurité (FDS)

Document complet couvrant les dangers, la manipulation, le stockage et la conformité réglementaire.

<<<<<<< HEAD Télécharger la SDS → ======= Télécharger la SDS → >>>>>>> release248-p3

✅ Certificat d'analyse (COA)

Données qualité spécifiques au lot. Saisissez votre numéro de lot pour récupérer le COA exact.

Rechercher le COA →

📊 Fiche technique

Résumé de référence rapide des spécifications et applications du produit.

Voir la fiche technique →

🔬 Fiche de spécifications

Attributs de qualité complets et critères d'acceptation pour cette qualité.

Voir la fiche de spécifications →

Données avancées

Cibles associées (humaines)
TNF Tclin Tumor necrosis factor (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificats (CoA, COO, BSE/TSE et tableau d'analyse)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
FAQ et articles
Calculateurs de solution
Avis

Avis des clients

Besoin d'aide pour choisir la qualité ?

Notre guide de sélection des qualités couvre la pureté, le statut du stabilisant et l'adéquation à l'application pour toutes les variantes de notre catalogue.

Voir le guide des qualités Animal Free → Voir le guide des qualités Carrier Free → Voir le guide des qualités Validated → Voir le guide des qualités ExactAb™ → Voir le guide des qualités Azide Free → Voir le guide des qualités High Performance → Voir le guide des qualités PBS Only →

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