TRIM5 alpha Mouse mAb - Anticorps primaire, specific to TRIM5 alpha

Cat. No.: Ab132527

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IP, WB

Host species

Souris

Reactive species

Humain

Isotype

Mouse IgG1

Clone Number

K94032_3D8

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human TRIM5 alpha Antibody, Monoclonal (K94032_3D8), could be used for WB, IP and so on.

Specifications

Product Name

TRIM5 alpha Mouse mAb - Anticorps primaire, specific to TRIM5 alpha

Synonymes

EC 6.3.2 | EC 6.3.2.- | RING finger protein 88 | RNF88 | RNF88tripartite motif protein TRIM | TRIM5 | TRIM5alpha | tripartite motif containing 5 | tripartite motif protein TRIM5 | tripartite motif-containing 5 | tripartite motif-containing protein 5 | RIN

Spécifications et pureté

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Mécanismes biochimiques et physiologiques

Capsid-specific restriction factor that prevents infection from non-host-adapted retroviruses. Blocks viral replication early in the life cycle, after viral entry but before reverse transcription. In addition to acting as a capsid-specific restriction fac

Espèce hôte

Souris

Spécificité

TRIM5 alpha

Immunogène

Recombinant protein of human TRIM5 (AA 31-270)

Contrôle positif

WB: Jurkat and HUVEC cell lysates. IP: Jurkat lysates.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Type de chaîne lumineuse

kappa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -80°C,Avoid repeated freezing and thawing

Expédié en

Dry ice packs + Cold packs

Stabilité et stockage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: TRIM5 alpha Mouse mAb (Ab132527)

TRIM5 alpha Mouse mAb (Ab132527) - Western Blot
All lanes: TRIM5 alpha Mouse mAb (Ab132527) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 57 kDa
Exposure time: 67.2 s

IP: TRIM5 alpha Mouse mAb (Ab132527)

TRIM5 alpha Mouse mAb (Ab132527) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of TRIM5 alpha Mouse mAb (Ab132527) with Jurkat lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. TRIM5 alpha Mouse mAb (Ab132527) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody.
Lane 1: Jurkat lysate
Lane 2: TRIM5 immunoprecipitated from Jurkat lysate by TRIM5 alpha Mouse mAb (Ab132527)
Lane 3: The isotype control antibody instead of TRIM5 alpha Mouse mAb (Ab132527) in HeLa lysate.
Result: TRIM5 alpha Mouse mAb (Ab132527) can immunoprecipitate TRIM5.

Application

• Application: Dilution info
• WB:

  1/1000 ;

• IP:

  1/100;

Cibles associées (humaines)

B3GNT6 Tbio Acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,3-N-acetylglucosaminyltransferase (0 Activities)

Discover Target: B3GNT6

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mécanismes d'action

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (TRIM5 alpha Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.