USH1C/Harmonin Mouse mAb - Anticorps primaire, specific to USH1C/Harmonin, Mouse IgG1

Cat. No.: Ab133668

GRADE & PURITY Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, WB

Host species

Souris

Reactive species

Humain, Souris

Isotype

Mouse IgG1

Clone Number

K06328_5A8

Conjugation

Unconjugated

Vue d’ensemble

Mouse anti Human USH1C/Harmonin Antibody, Monoclonal (K06328_5A8), could be used for WB, IHC and so on.
Application:
WB: 1/1000
IHC: 1/1000
Protein Function:
May be involved in protein-protein interaction.

Specifications

Product Name

USH1C/Harmonin Mouse mAb - Anticorps primaire, specific to USH1C/Harmonin, Mouse IgG1

Synonymes

AIE 75antibody | AIE75 antibody | Antigen NY CO 38/NY CO 37 antibody | Antigen NY-CO-38/NY-CO-37 antibody | Autoimmune enteropathy related antigen AIE 75 antibody | Autoimmune enteropathy related antigen AIE75 antibody | Autoimmune enteropathy-related ant

Spécifications et pureté

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Espèce hôte

Souris

Spécificité

USH1C/Harmonin

Immunogène

Recombinant protein of human USH1C/Harmonin (AA 301-550).

Contrôle positif

WB: Mouse kidney lysates. IHC: Human small intestine tissue.

Conjugation

Unconjugated

Grade

Carrier Free, ExactAb™, Validated

Isotype

Mouse IgG1

Type de chaîne lumineuse

kappa

SDS-PAGE

150 kDa

Méthode de purification

Protein A purified

Concentration

1.0 mg/mL

Product Line

Produits biologiques

Stockage et expédition

Forme

Liquid

Conditions de stockage de stockage

Store at -80°C,Avoid repeated freezing and thawing

Expédié en

Dry ice packs + Cold packs

Stabilité et stockage

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

Western Blot: USH1C/Harmonin Mouse mAb (Ab133668)

USH1C/Harmonin Mouse mAb (Ab133668) - Western Blot
All lanes: USH1C/Harmonin Mouse mAb (Ab133668) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 62 kDa
Observed band size: 67 kDa
Exposure time: 100.0 s

IHC: USH1C/Harmonin Mouse mAb (Ab133668)

USH1C/Harmonin Mouse mAb (Ab133668) - IHC
IHC analysis of small intestine tissue by USH1C/Harmonin Mouse mAb (Ab133668).IHC was performed using sections of the formalin-fixed paraffin-embedded small intestine tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H₂O₂ for 30 min at room temperature. The sections were then incubated with USH1C/Harmonin Mouse mAb (Ab133668) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Glandular cells are positively stained at the cytoplasm and membrane.

Application

Application

Dilution info

WB

1/1000

IHC

1/1000

Cibles associées (humaines)

USH1C Tbio Harmonin (0 Activities)

Discover Target: USH1C

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

• Tested applications, recommended dilutions, and positive control samples: Not specified for this item; refer to CoA/Spec Sheet.
• General guidance (literature/common practice; confirm suitability for this item)
  • Western blot: Start 1:500–1:2,000; optimize by checkerboard titration.
  • IF/ICC: Start 1–5 μg/mL or 1:200–1:800 depending on detection system and fixation.
  • IHC: Optimize antigen retrieval and blocking; start 1–5 μg/mL.
  • IP: 1–5 μg antibody per mg lysate protein as an initial condition.
  • Include appropriate controls: no-primary, isotype control, and known-positive lysates/tissues.
• Note: The above are general starting points. Use the CoA/Spec Sheet for item-specific, validated protocols if available.

Biological Roles

• Target overview (literature/general)
  • Harmonin is a scaffold protein encoded by the USH1C gene. It contains multiple PDZ domains and coiled-coil regions that coordinate protein–protein interactions within specialized sensory structures.
  • Cellular localization: Predominantly associated with actin-rich structures such as stereocilia of inner ear hair cells and also reported at cell junctions and photoreceptor-related compartments in various species. Localization can depend on isoform and developmental stage.
  • Functional themes: Organizes multi-protein complexes by binding to PDZ-binding motifs of partner proteins; contributes to cytoskeletal linkage and mechanotransductive architecture.
  • Isoforms: Alternative splicing yields several harmonin isoforms (a, b, c classes) that differ in domain composition, subcellular targeting, and interaction partners.
• Research utility of anti-harmonin antibodies (general)
  • Mapping expression patterns across tissues and developmental time courses.
  • Studying assembly of protein complexes through IP/Co-IP and proximity assays.
  • Validating genetic perturbations (e.g., siRNA/CRISPR) at the protein level.
• Notes for experimental design
  • Epitope availability can vary between isoforms and fixation conditions; verify whether the antibody recognizes a shared or isoform-specific epitope (not specified for this item).
  • Cross-species reactivity and specificity should be established empirically for the system of interest (not specified for this item).

Buffer Applications

• Not typically applicable.
• As an antibody reagent, this product is not used to prepare buffer systems. Use standard antibody-compatible buffers (e.g., PBS, TBS, with appropriate blocking agents) tailored to the chosen assay. Composition and pH for this item are not specified; refer to CoA/Spec Sheet.

Green Alternatives

• Environmental considerations for antibody use (general)
  • Cold-chain logistics: Shipping on dry ice and storage at −80°C are energy-intensive. Consolidate orders and minimize shipments to reduce carbon footprint.
  • Stabilization strategies: Where permissible and consistent with experimental needs, aliquoting to reduce freeze–thaw and storing at −20°C or 4°C short-term can decrease deep-freeze time (follow item-specific guidance; this product specifies −80°C storage).
  • Buffer choices: Use phosphate or Tris buffers prepared with purified water; avoid azide where not required to reduce hazardous waste.
• Comparison of handling options (general; not product specs)
  • −80°C storage: Maximizes long-term stability; highest energy use.
  • −20°C storage: Suitable for some antibodies with stabilizers; lower energy use but may not preserve activity long-term.
  • Lyophilized formats: Often more transport-stable and lower footprint; reconstitution adds steps. This item’s physical state is not specified.
• Waste minimization
  • Use low-binding tubes to reduce losses and repeats.
  • Plan experiments to fully utilize aliquots and prevent partial refreezing.
• Note: Always prioritize item-specific instructions. For this product, storage at −80°C and avoiding repeated freeze–thaw are specified.

Pharmaceutical Uses

• Not intended for pharmaceutical or clinical use.
• Research/formulation context (general)
  • Carrier-free monoclonal antibodies are often selected for custom conjugation (enzymes, fluorophores, affinity tags) to build analytical reagents for diagnostics research and bioassays.
  • No pharmacopeial status, excipients, or GMP attributes are provided for this item. Do not use in human or veterinary applications.
• Statement of use
  • For research use only (as specified).

Physical Properties

• Item-specific values
  • Appearance, concentration, buffer composition, pH, and excipients: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight (actual for this clone): Not specified for this item; refer to CoA/Spec Sheet.
• General/literature values for monoclonal IgG (for context only; not product specifications)
  • Typical molecular weight: ~150 kDa for full-length IgG; ~50 kDa heavy chain and ~25 kDa light chain under reducing SDS-PAGE.
  • Isoelectric point (pI): Often in the range 7–9, but highly sequence-dependent.
  • Solubility: Highly soluble in aqueous physiological buffers (e.g., PBS, TBS) when properly formulated; sensitive to extremes of pH and to organic solvents.
  • Stability considerations: Enhanced by neutral pH, low temperature, and stabilizers (e.g., sugars, proteins) though this item is carrier-free per listing; actual excipients not specified.
  • Absorbance: A280 commonly used for quantitation; extinction coefficient is antibody- and sequence-specific (not specified for this item).
• Practical notes
  • Avoid vigorous agitation and repeated freeze–thaw cycles to minimize aggregation.
  • Filter sterilization (0.22 μm) can be used if bioburden control is needed, recognizing potential minimal loss to membranes.

Quality and Grades

• Item-specific quality designations
  • Carrier Free: Indicates the antibody is supplied without common carrier/stabilizer proteins (e.g., BSA), glycerol, or azide, improving compatibility with conjugation and sensitive assays. Exact excipient profile and concentration are not specified for this item; refer to CoA/Spec Sheet.
  • ExactAb™: Manufacturer branding denoting a defined monoclonal preparation with rigorous characterization. Specific production modality and validation parameters are not specified for this item; consult CoA/Spec Sheet for details.
  • Validated: The product has been qualified by the manufacturer; however, specific tested applications, species reactivity, and dilution ranges are not provided here and should be confirmed on the CoA/Spec Sheet.
• Practical implications
  • Conjugation-ready: Carrier-free format minimizes background and eliminates the need for carrier removal prior to biotin, enzyme, or fluorophore labeling.
  • Reproducibility: Monoclonal format supports lot-to-lot consistency; verify epitope/clone information before critical experiments (not specified here).
  • Downstream compatibility: Absence of carriers typically benefits mass spectrometry, live-cell work, and functional assays where additives can interfere.
• Documentation
  • Certificate of Analysis (CoA)/Specification Sheet should be used to confirm: clone ID, isotype, concentration (mg/mL), purity method, endotoxin level, sterility status, and validated applications. Where values are not present above, they are not specified for this item and must be verified in the CoA.

Reaction and Applications

• Context
  • This is a mouse monoclonal antibody specific for USH1C/harmonin, intended for research use.
• Typical antibody applications (literature/general; confirm suitability on CoA for this specific item)
  • Western blot (WB): Detection of denatured epitopes in reduced or non-reduced lysates; optimize transfer and blocking (casein or BSA) to minimize background.
  • Immunoprecipitation (IP)/Co-IP: Capture native complexes; pre-clear lysates, use balanced salt buffers, and include protease inhibitors.
  • Immunofluorescence (IF)/Immunocytochemistry (ICC): Fixation and permeabilization conditions (e.g., PFA + Triton X-100) must preserve harmonin epitopes; include no-primary controls.
  • Immunohistochemistry (IHC): Antigen retrieval may be required for formalin-fixed tissues; optimize retrieval buffer pH (citrate vs Tris-EDTA) empirically.
  • ELISA: Use carrier-free antibody for coating or detection; ensure orientation via Protein A/G capture where appropriate.
• Practical tips
  • Titering: Perform dilution series to determine working concentration; avoid over-saturation to preserve specificity.
  • Species considerations: When probing mouse tissues with mouse mAb, consider anti-mouse background and use Fab fragment secondary or mouse-on-mouse kits.
  • Secondary antibodies: Select anti-mouse secondary matched to the isotype (isotype not specified here—verify on CoA).
• Note: Specific validated applications and recommended dilutions for this item are not specified; refer to CoA/Spec Sheet.

Reaction Conditions

• Not applicable to chemical reactions.
• For immunoassays, conditions are assay-specific and should be optimized (e.g., antibody concentration, incubation time/temperature, buffer composition). No item-specific conditions are provided; refer to CoA/Spec Sheet and standard immunoassay protocols.

Safety and Handling

• Hazard information (item-specific)
  • GHS classification, pictograms, H-statements, signal word: Not specified for this item; consult the product SDS for authoritative safety guidance.
• General safety for antibody reagents (literature/general)
  • Typical hazard profile: Protein aqueous solutions are generally considered of low acute toxicity; however, preservatives (e.g., sodium azide) or residual process reagents may present hazards—composition for this item is not specified.
  • PPE: Standard laboratory PPE is recommended—lab coat, disposable nitrile gloves, and splash-resistant eye protection.
  • Handling: Work on ice or at 2–8°C when setting up experiments. Avoid creating aerosols. Use low-binding plastics to reduce adsorption.
  • Incompatibilities: Avoid strong oxidizers, proteases, extreme pH, and organic solvents that denature proteins.
• First-aid overview (general)
  • Skin/eye contact: Rinse with water for several minutes; remove contaminated clothing. Seek medical evaluation if irritation persists.
  • Ingestion/inhalation: Rinse mouth; move to fresh air if aerosol was generated. Obtain medical attention if unwell.
• Special risks and good practices
  • Freeze–thaw sensitivity: Repeated cycles promote aggregation and loss of activity—aliquot upon first thaw.
  • Bioburden: Maintain aseptic technique if sterility is required for cell-based assays.
  • Disposal: Follow institutional procedures for proteinaceous laboratory waste; account for any preservatives per SDS.

Solvent Selection

• Not typically applicable.
• This product is a protein biologic supplied in an aqueous buffer. Do not dilute into organic solvents; maintain in compatible physiological buffers (e.g., PBS/TBS) as per CoA. For conjugation workflows, use additive-free buffers at recommended pH and ionic strength.

Storage and Reconstitution

• Item-specific instructions
  • Storage: Store at −80°C (as specified). Avoid repeated freezing and thawing.
  • Shipping: Shipped on dry ice packs + cold packs.
• Practical handling
  • Upon receipt: Immediately transfer to −80°C. If frequent use is anticipated, aliquot into low-binding microtubes under sterile conditions to minimize freeze–thaw.
  • Thawing: Thaw on ice. Mix gently by inversion; avoid vortexing to prevent aggregation.
  • Short-term storage: Keep on ice during use. For interim storage between experiments, many labs hold aliquots at 4°C for ≤1–2 days; longer-term stability must follow the specified −80°C storage.
• Reconstitution
  • Physical state and recommended reconstitution buffer are not specified for this item; refer to CoA/Spec Sheet. For carrier-free antibodies in general, reconstitution (if lyophilized) is commonly performed in sterile PBS or an additive-free buffer appropriate for planned conjugation/assays.
• Additional notes
  • Do not expose to repeated freeze–thaw cycles; plan aliquot sizes according to expected single-use needs.
  • Buffer composition, concentration, and any preservatives/excipients are not specified for this item and should be confirmed on the CoA/Spec Sheet.
  • Maintain documentation of aliquot dates and storage conditions for reproducibility.

Structure and Identity

• Item-specific identifiers
  • Product name: USH1C/Harmonin Mouse mAb (SKU: Ab133668)
  • Target: USH1C (harmonin) protein
  • Grade/Purity: Carrier Free; ExactAb™; Validated
  • CAS, CID, InChIKey, SMILES: Not applicable for monoclonal antibodies; not specified for this item; refer to CoA/Spec Sheet.
• Molecular information (item-specific)
  • Molecular formula: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular weight: Not specified for this item; refer to CoA/Spec Sheet.
• Structural features (general for a mouse monoclonal antibody; literature/general)
  • Typical architecture: IgG-class immunoglobulin composed of two heavy and two light polypeptide chains, forming a Y-shaped glycoprotein (~150 kDa total for a full-length IgG).
  • Domain organization: Variable domains (VH/VL) confer antigen recognition; constant domains (CH/CL) define isotype and effector functions. Fc region mediates secondary interactions with Protein A/G and Fc receptors.
  • Antigen-binding site: Complementarity-determining regions (CDRs) in VH/VL form a paratope specific for harmonin epitopes.
  • Post-translational features: N-linked Fc glycosylation typical for IgG (composition and glycoform distribution item-specific; not specified).
• 2D structure description (general)
  • A central Fc stem (CH2–CH3 dimer) with two Fab arms (each containing one VH–VL pair) connected via a flexible hinge; disulfide bonds link heavy–heavy and heavy–light chains.
• Clone, isotype, light-chain type, sequence
  • Not specified for this item; refer to CoA/Spec Sheet.

Synthetic Utility

• Not applicable.
• This is a biological reagent (monoclonal antibody), not a synthetic building block or small-molecule reagent. For chemical synthesis applications, refer instead to small-molecule catalysts, ligands, or substrates appropriate to the reaction type.

Target Specificity

• Antigen/Target name: USH1C/Harmonin (from product name)
• Epitope: Not specified for this item; refer to CoA/Spec Sheet.
• Clone ID: Not specified for this item; refer to CoA/Spec Sheet.
• Isotype/Light chain: Not specified for this item; refer to CoA/Spec Sheet.
• Species reactivity/cross-reactivity: Not specified for this item; refer to CoA/Spec Sheet.
• Validations: Listed as “Validated,” but specific applications and conditions are not provided here; consult CoA/Spec Sheet.

Foire aux questions

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (USH1C/Harmonin Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.