Preparation of media with dry powder
Dissolve all the dry powder in the package with appropriate amount of ultrapure water: put the container on the magnetic stirrer and add the dry powder while st
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Experiment on the determination of protein content by Bradford's method
Dissolve proteins, mix with dye, and read O.D. after 10 min. Source: Animal Cell Culture: A Guide to Basic Techniques, Fifth Edition
Protein synthesis experiments
The colorimetric method can determine the amount of total protein at any point in time. Continuous observation over a period of time can be used to determine ne
Isozyme analysis
Cells were collected, washed with D-PBSA and reconstituted into a cell suspension of 5×107 cells/ml, cell extracts were prepared and stored at -70°C.
indirect immunofluorescence
The cells were fixed and primary and secondary antibodies were added sequentially and visualized by UV light.
Preparation of special media
Prepare storage concentrates and freeze for preservation. Thaw, mix, aseptically filter and store when needed.
Vacuum filter bottle sterilization and filtration experiment
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Fluorescence detection of Mycoplasma
Cells were cultured for at least one week in the absence of antibiotics, culture supernatants were transferred to the indicated cells in early logarithmic growt
Experiments for plotting growth curves of suspension-cultured cells
A series of cell cultures was performed at three different cell concentrations and cells were counted daily until plateau. Source: Animal Cell Culture: A Guide