Isolation of lymphocytes
After removal of erythrocytes by dextran-promoted sedimentation, a citrate- or heparin-anticoagulated layer of whole blood or plasma is added on top of the dens
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Sterilization and filtration with large tandem filters
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Sterilization and filtration with small tandem filters
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
PCR Determination of Mycoplasma
This lab was derived from: Animal Cell Culture - A Guide to Basic Techniques (5th Edition)
Measurement experiments of wall affixation efficiency
Inoculate cells at low density, incubate until colonies form, stain and count colonies Source : Animal Cell Culture : A Guide to Basic Techniques 5th Edition
human biopsy tissue
In consultation with hospital personnel, provide labeled containers with culture media and arrange to have samples taken from the operating room or pathologist.
Serum collection and sterilization
Blood is collected, allowed to clot, and the serum is separated. Filter the serum through filters with progressively decreasing pore sizes. Dispense vials, pack
Warm trypsin dissociation of tissues
The tissue was minced and stirred in trypsin for several hours, and the isolated cells were collected every half hour, centrifuged, and added to serum-containin
prostate epithelium
The prostate was removed and the specimen processed (30 min), and the prostate tissue was digested with collagenase (1 h) to collect individual cells and small