BDNF Antibody - Primary antibody, specific to BDNF, Rabbit IgG

Cat. No.: Ab090903

GRADE & PURITY Validated, ExactAb™, 1.0 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human BDNF Antibody, Polyclonal, could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

BDNF Antibody - Primary antibody, specific to BDNF, Rabbit IgG

Sinónimos

BDNF | ANON2 | BULN2 | Abrineurin antibody | ANON2 antibody | BDNF antibody | BDNF_HUMAN antibody | Brain Derived Neurotrophic Factor antibody | Brain-derived neurotrophic factor antibody | BULN2 antibody | MGC34632antibody | Neurotrophinantibody | BDNF R

Especificações e pureza

ExactAb™, Validated, 1.0 mg/mL

Mecanismos bioquímicos e fisiológicos

During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regula

Espécie hospedeira

Rabbit

Especificidade

BDNF

Imunogénio

A synthetic peptide derived from human BDNF (AA 150-200).

Controlo positivo

WB: Mouse heart, HeLa, HEK293, THP-1, K562, Hep G2, RAW 264.7 and C6 lysates. IHC: Human brain tissue. IF/ICC: HeLa cell.

Conjugação

Unconjugated

Grau

ExactAb™, Validated

Isótipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificação

Protein A purified

Concentração

1.0 mg/mL

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: BDNF Antibody (Ab090903)

BDNF Antibody (Ab090903) - Western Blot
All lanes: BDNF Antibody (Ab090903) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 17, 28, 30 kDa
BDNF is synthesized as a precursor form (pro-BDNF) and is converted into its active form through proteolytic processing. The precursor protein is first cleaved to pro-BDNF (~35 kDa) by removing a signal peptide, and then to a mature form of BDNF (~15 kDa). Additionally, pro-BDNF can form dimers that run between 50 -60 kDa, and a minor truncated form of pro-BDNF running at ~28 kDa has also been reported (PMID: 11152678, 20630543).
Exposure time: 3.2 s

Western Blot: BDNF Antibody (Ab090903)

BDNF Antibody (Ab090903) - Western Blot
All lanes: BDNF Antibody (Ab090903) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 28 kDa
Observed band size: 16, 28, 30, 57 kDa
BDNF is synthesized as a precursor form (pro-BDNF) and is converted into its active form through proteolytic processing. The precursor protein is first cleaved to pro-BDNF (~35 kDa) by removing a signal peptide, and then to a mature form of BDNF (~15 kDa). Additionally, pro-BDNF can form dimers that run between 50 -60 kDa, and a minor truncated form of pro-BDNF running at ~28 kDa has also been reported (PMID: 11152678, 20630543).
Exposure time: 44.7 s
 

IF/ICC: BDNF Antibody (Ab090903)

BDNF Antibody (Ab090903) - IF/ICC
Immunofluorescence analysis of BDNF in HeLa using BDNF Antibody (Ab090903), and cells were counterstained with DAPI (blue).

IHC: BDNF Antibody (Ab090903)

BDNF Antibody (Ab090903) - IHC
Immunohistochemistry analysis of paraffin-embedded Human brain using BDNF Antibody (Ab090903). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Aplicação

• Aplicação: Dilution info
• IHC:

  1/100 - 1/200;

• IF/ICC:

  1/50-1/200 ;

• WB:

  1/1000 - 1/2000

Alvos associados (humanos)

BDNF Tchem Brain-derived neurotrophic factor (0 Activities)

Discover Target: BDNF

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação