PD1 Mouse mAb (Biotin) - Primary antibody, specific to PD1, Mouse IgG1

Cat. No.: Ab179528

GRADE & PURITY ExactAb™, Validated, 0.5 mg/mL

Application

Flow Cytometry

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG1

Clone Number

EH12.2H7

Conjugation

Biotin

Visão geral

Application:
Flow: ≤0.5 μg/test
For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume or 100 µL of whole blood.

Specifications

Product Name

PD1 Mouse mAb (Biotin) - Primary antibody, specific to PD1, Mouse IgG1

Sinónimos

CD279 | hPD-1 | PD1 | PD-1 | PD1hPD-l | PDCD1 | programmed cell death 1 | programmed cell death protein 1 | Protein PD-1 | SLEB2 | CD279 antibody | CD279 antigen antibody | hPD 1 antibody | CD279 antigen | hPD l antibody | hPD-1 antibody | hSLE1 antibody

Especificações e pureza

ExactAb™, Validated, 0.5 mg/mL

Espécie hospedeira

Mouse

Especificidade

PD1

Imunogénio

Recombinant protein of human CD279

Controlo positivo

Flow: PMA and lonomycin stimulated (overnight) MOLT4

Conjugação

Biotin

Grau

ExactAb™, Validated

Isótipo

Mouse IgG1

Tipo de corrente de luz

kappa

Método de purificação

Protein G purified

Concentração

0.5 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at 2-8°C,Do not freeze

Enviado em

Wet ice,Do not freeze

Estabilidade e armazenamento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imagens

Flow Cytometry: PD1 Mouse mAb (Biotin) (Ab179528)

PD1 Mouse mAb (Biotin) (Ab179528) - Flow Cytometry
MOLT-4 cells treated with 500 ng/mL Ionomycin and 10 ng/mL Phorbol-12-myristate-13-acetate (PMA) for 24 hours (right panel) are stained with PD1 Mouse mAb (Biotin) (Ab179528) (red) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody. Untreated MOLT-4 cells were used as control (left panel).

Aplicação

Aplicação

Dilution info

Flow Cytometry

≤0.5μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.5 μg per million cells in 100 µL staining volume.

Alvos associados (humanos)

PDCD1 Tclin Programmed cell death protein 1 (0 Activities)

Discover Target: PDCD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

PDCD1 Tclin Programmed cell death protein 1 (14 Activities)

Discover Target: PDCD1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PD1 Mouse mAb (Biotin)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.