Profilin 1 Mouse mAb - Primary antibody, specific to Profilin 1, Mouse IgG2b

Cat. No.: Ab183903

GRAU & PUREZA Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

Flow Cytometry, WB

Host species

Mouse

Reactive species

Human

Isotype

Mouse IgG2b

Clone Number

AB02/3H12

Conjugation

Unconjugated

Visão geral

Mouse anti Human Profilin 1 Antibody, clone AB02/3H12, could be used for WB, Flow, ELISA and so on.

Especificações

Product Name

Profilin 1 Mouse mAb - Primary antibody, specific to Profilin 1, Mouse IgG2b

Sinónimos

Actin binding protein antibody | ALS18 antibody | Epididymis tissue protein Li 184a antibody | OTTHUMP00000125244 antibody | PFN 1 antibody | Pfn antibody | PFN1 antibody | PROF1_HUMAN antibody | Profilin I antibody | Profilin-1 antibody | Profilin1 antib

Especificações e pureza

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Mecanismos bioquímicos e fisiológicos

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Inhibits androg

Espécie hospedeira

Mouse

Especificidade

Profilin 1

Imunogénio

Recombinant protein corresponding to Human Profilin 1

Controlo positivo

WB: HeLa, HepG2, HT-29, K562, HEK293, A431 and Jurkat whole cell lysates Flow: HeLa cells

Conjugação

Unconjugated

Grau

Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Isótipo

Mouse IgG2b

Tipo de corrente de luz

lambda

SDS-PAGE

150.0 KDa

Método de purificação

Protein A purified

Concentração

1.0 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Imagens

Western Blot: Profilin 1 Mouse mAb (Ab183903)

Profilin 1 Mouse mAb (Ab183903) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab183903) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 16 kDa
Exposure time: 54.0 s 

Flow Cytometry: Profilin 1 Mouse mAb (Ab183903)

Profilin 1 Mouse mAb (Ab183903) - Flow Cytometry
Flow Cytometry analysis of HeLa cells stained with Profilin 1 Mouse mAb (Ab183903) (Red) at 2.5 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).

Western Blot: Profilin 1 Mouse mAb (Ab183903)

Profilin 1 Mouse mAb (Ab183903) - Western Blot
All lanes: Profilin 1 Mouse Antibody (Ab183903) at 1/10000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 15.0 s 

Aplicação

Aplicação

Dilution info

Flow Cytometry

1/200 - 1/50,000

WB

1/1,000 - 1/100,000; Predicted molecular weight: 15.0 KDa

Alvos associados (humanos)

PFN1 Tbio Profilin-1 (0 Activities)

Discover Target: PFN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Perguntas frequentes

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Profilin 1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.