Recombinant MAP1LC3A Antibody - Primary antibody, specific to MAP1LC3A, Rabbit IgG

Cat. No.: Ab114168

GRADE & PURITY Recombinant, ExactAb™, Validated, 0.3 mg/mL

Application

IF/ICC, IHC, WB

Host species

Rabbit

Reactive species

Human, Mouse, Rat

Isotype

Rabbit IgG

Conjugation

Unconjugated

Visão geral

Rabbit anti Human MAP1LC3A Antibody, Recombinant, could be used for WB, IHC and so on.

Application:

WB: 1/500-1/1000 

IHC: 1/50-1/100

IF/ICC: 1/200

Protein Function

Probably involved in formation of autophagosomal vacuoles (autophagosomes).

Specifications

Product Name

Recombinant MAP1LC3A Antibody - Primary antibody, specific to MAP1LC3A, Rabbit IgG

Sinónimos

ATG8E antibody | Autophagy related protein LC3 A antibody | Autophagy related ubiquitin like modifier LC3 A antibody | Autophagy-related protein LC3 A antibody | Autophagy-related ubiquitin-like modifier LC3 A antibody | LC3 antibody | LC3A antibody | MAP

Especificações e pureza

ExactAb™, Validated, Recombinant, 0.3 mg/mL

Espécie hospedeira

Rabbit

Especificidade

MAP1LC3A

Imunogénio

A synthetic peptide derived from human MAP1LC3A (AA 101-120).

Controlo positivo

WB: NIH/3T3, HeLa, C6, Mouse heart, Mouse brain lysates. IHC: Human breast cancer tissue. IF/ICC: HeLa cells.

Conjugação

Unconjugated

Grau

ExactAb™, Recombinant, Validated

Isótipo

Rabbit IgG

SDS-PAGE

150 kDa

Método de purificação

Antigen affinity purified

Concentração

0.3 mg/mL

Product Line

Produtos biológicos

Armazenamento e envio

Forma

Liquid

Condições de armazenamento de armazenamento

Store at -20°C,Avoid repeated freezing and thawing

Enviado em

Ice chest + Ice pads

Estabilidade e armazenamento

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Imagens

Western Blot: Recombinant MAP1LC3A Antibody (Ab114168)

Recombinant MAP1LC3A Antibody (Ab114168) - Western Blot
All lanes: Recombinant MAP1LC3A Antibody (Ab114168) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution

Predicted band size: 14 kDa
Observed band size: 14, 16 kDa
Exposure time: 57.2 s

IHC: Recombinant MAP1LC3A Antibody (Ab114168)

Recombinant MAP1LC3A Antibody (Ab114168) - IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant MAP1LC3A Antibody (Ab114168). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

IF/ICC: Recombinant MAP1LC3A Antibody (Ab114168)

Recombinant MAP1LC3A Antibody (Ab114168) - IF/ICC

IF analysis of Recombinant MAP1LC3A Antibody (Ab114168) in HeLa cells. The cells were fixed with 4% PFA for 10 minutes, permeabilized with 0.3% Triton X-100 (I743383) for 10 minutes and then blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant MAP1LC3A Antibody (Ab114168) at 1/200 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Rabbit IgG H&L (AF488) (Ab181954) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). Cells were counterstained with DAPI (blue).

Aplicação

Aplicação

Dilution info

IHC

1/50 - 1/100

IF/ICC

1/200

Alvos associados (humanos)

MAP1LC3A Tbio Microtubule-associated proteins 1A/1B light chain 3A (0 Activities)

Discover Target: MAP1LC3A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Mecanismos de ação

Perguntas frequentes

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Recombinant mean?

Recombinant is a defined analytical purity tier suitable for workflows requiring a specific validated property. It specifies a minimum assay percentage, controlled limits on common impurities (water, heavy metals, residue on ignition), and lot-to-lot consistency. Recombinant is one of several purity tiers — choose based on the sensitivity and regulatory requirements of your method.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Recombinant MAP1LC3A Antibody); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.