GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.KD Validation?Knockdown-validated — specificity supported by signal loss after target knockdown. Use when you want functional evidence of antibody specificity.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.See COA
Store at -20°C,Avoid repeated freezing and thawing
Enviado en
Ice chest + Ice pads
Estabilidad y almacenamiento
Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Imágenes
CEP55 Mouse mAb (Ab336926) - Western Blot
Centrosomal protein 55 expression in wild-type (WT) and centrosomal protein 55 (CEP55) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CEP55 Mouse mAb (Ab336926) (1/2500) and Goat Anti-Mouse IgG H&L (HRP) (Ab179001) (1/20000) respectively.
CEP55 Mouse mAb (Ab336926) - IF/ICC
Immunofluorescent analysis of HeLa cells using CEP55 Mouse mAb (Ab336926) at 1/1000 dilution. Top panel: wild-type (WT); Bottom panal: CEP55 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Centrosomal protein 55 was stained magenta with Alexa Fluor® 647. Fixative solution: 4% Paraformaldehyde in 1xPBS; Permeabilization solution: 0.5% Triton X-100 in 1xPBS. Scale bar, 20 μm.
CEP55 Mouse mAb (Ab336926) - Flow Cytometry
Flow cytometric analysis of Centrosomal protein 55 expression in HeLa cells using CEP55 Mouse mAb (Ab336926) at 1/1000 dilution. Fixative solution: 4% Paraformaldehyde in 1xPBS; Permeabilization solution: 0.5% Triton X-100 in 1xPBS. Green, isotype control; red, Centrosomal protein 55.
CEP55 Mouse mAb (Ab336926) - Western Blot
All lanes: CEP55 Mouse mAb (Ab336926) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 55 kDa
Observed band size: 56 kDa
Exposure time: 125.5 s
Aplicación
Aplicación
Dilution info
Flow Cytometry
1/100-1/1000
IF/ICC
1/100-1/1000
WB
1/500-1/2500
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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