Cyanine 3 Tyramide - ≥95%(HPLC) , CAS No.174961-75-2

CAS: 174961-75-2 Cat. No.: C287079 Peso molecular: 749.94 PubChem CID: 146018932

GRADE & PURITY ≥95%(HPLC)

Synonyms

2-[3-[(1-Ethyl-1,3-dihydro-3,3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3,3-dimethyl-5-sulfo-3H-indolium inner salt | Tyramide-Cy3

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Descripción general

Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids

Application：

Tyramide signal amplification (TSA) in IHC, ICC, ISH, FISH

Spectral characteristics:

Emission Color                            Orange

λabs                                        550 nm

λem                                        563 nm

Extinction Coefficient (ε)           150000 M-1cm-1

Quantum Yield (φ)                0.31

Closest Laser line                532 nm

Specifications

Sinónimos

2-[3-[(1-Ethyl-1, 3-dihydro-3, 3-dimethyl-5-sulfo-2H-indol-2-ylidene)-1-propen-1-yl]-1-[6-[[2-(4-hydroxyphenyl)ethyl]amino-6-oxohexyl]-3, 3-dimethyl-5-sulfo-3H-indolium inner salt | Tyramide-Cy3

Especificaciones y pureza

≥95%(HPLC)

Mecanismos bioquímicos y fisiológicos

Orange fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. HRP catalyzes localized deposition of multiple tyramide molecules (catalyzed reporter deposition, CARD), binding the fluorescein tyramide

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

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Pureza

≥95%(HPLC)

Nombres e identificadores

• Pubchem Sid: 504773614
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504773614
• Sonrisas canónicas: CCN1C2=C(C=C(C=C2)S(=O)(=O)O)C(C1=CC=CC3=[N+](C4=C(C3(C)C)C=C(C=C4)S(=O)(=O)[O-])CCCCCC(=O)NCCC5=CC=C(C=C5)O)(C)C
• IUPAC Name: 2-[(E,3E)-3-(1-ethyl-3,3-dimethyl-5-sulfoindol-2-ylidene)prop-1-enyl]-1-[6-[2-(4-hydroxyphenyl)ethylamino]-6-oxohexyl]-3,3-dimethylindol-1-ium-5-sulfonate
• InChIKey: JPXIOSIRIWBDIC-UHFFFAOYSA-N
• INCHI: 1S/C39H47N3O8S2/c1-6-41-33-20-18-29(51(45,46)47)25-31(33)38(2,3)35(41)11-10-12-36-39(4,5)32-26-30(52(48,49)50)19-21-34(32)42(36)24-9-7-8-13-37(44)40-23-22-27-14-16-28(43)17-15-27/h10-12,14-21,25-26H,6-9,13,22-24H2,1-5H3,(H3-,40,43,44,45,46,47,48,49,50)
• PubChem CID: 146018932
• Peso molecular: 749.94

Propiedades químicas y físicas

• Solubilidad: Solvent:DMSO, Max Conc. mg/mL: 12, Max Conc. mM: 16