D-Luciferin, Sodium Salt - Suitable for molecular biology, ≥99.9%(HPLC) , CAS No.103404-75-7

CAS: 103404-75-7 Cat. No.: D1375459 Peso molecular: 302.3 Número EC: 600-430-4

GRADE & PURITY Suitable for molecular biology, ≥99.9%(HPLC)

Synonyms

D-Luciferin | Sodium Salt monohydrate | (S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid sodium salt | 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt | Firefly luciferin sodium salt | D-Luciferin (sodium

Storage

Protected from light,Store at -20°C,Desiccated

Shipped In

Ice chest + Ice pads

Application

ATP Assay, Bioluminescence, Cell Viability Assay, In Vivo Imaging, Reporter Gene Assay

Descripción general

  Luciferin is a common bioluminescent reporter used for in vivo imaging of the expression of luciferase. This water soluble substrate for the firefly luciferase enzyme utilizes ATP and Mg²⁺ as cofactors to emit a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Through the utilization of ATP, the reaction can be further used to indicate the presence of energy or life in order to function as a life-death stain.

  Luciferin is a common reagent used throughout the biotechnology field and specifically for in vivo imaging. Luciferase labeled tumor cells, stem cells or infectious diseases are often inoculated into research animals such as rats or mice for investigation. The injection of luciferin allows for the real-time, noninvasive monitoring of disease progression and/or drug efficacy in these model systems through Bioluminescence Imaging (BLI).

  Luciferin is also commonly used for in vitro research, including luciferase and ATP assays, gene reporter assays, high throughput sequencing and various contamination assays.
APPLICATIONS

in vivo cellular imaging

luciferase and ATP assays

gene reporter assays

high throughput sequencing

contamination assays
Assay Buffer

Tris-MgCl₂-CoA 缓冲液	Final Concentration
Tris-HCl, pH 7.8	100mM
MgCl₂	5mM
Coenzyme A (CoA) (hydrate)	0.25mM
ATP (disodium salt hydrate)	0.15mM

Specifications

Sinónimos

D-Luciferin | Sodium Salt monohydrate | (S)-2-(6-Hydroxy-2-benzothiazolyl)-2-thiazoline-4-carboxylic acid sodium salt | 4, 5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid sodium salt | Firefly luciferin sodium salt | D-Luciferin (sodium

Especificaciones y pureza

Suitable for molecular biology, ≥99.9%(HPLC)

Estabilidad y almacenamiento

Store at -20℃ long term (5 years). Store in the dark. Desiccated.

Condiciones de almacenamiento de almacenamiento

Protected from light, Store at -20°C, Desiccated

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

Suitable for molecular biology

Pureza

≥99.9%(HPLC)

Nombres e identificadores

• Sonrisas canónicas: C1C(N=C(S1)C2=NC3=C(S2)C=C(C=C3)O)C(=O)[O-].[Na+]
• IUPAC Name: sodium;(4S)-2-(6-hydroxy-1,3-benzothiazol-2-yl)-4,5-dihydro-1,3-thiazole-4-carboxylate
• InChIKey: LILQLBIQROYWIA-OGFXRTJISA-M
• INCHI: 1S/C11H8N2O3S2.Na/c14-5-1-2-6-8(3-5)18-10(12-6)9-13-7(4-17-9)11(15)16;/h1-3,7,14H,4H2,(H,15,16);/q;+1/p-1/t7-;/m1./s1
• Isómeros SMILES: C1[C@@H](N=C(S1)C2=NC3=C(S2)C=C(C=C3)O)C(=O)[O-].[Na+]
• WGK Alemania: 3
• Peso molecular: 302.3
• Reaxy-Rn: 62728073
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=62728073&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Clase Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Amino acids and derivatives
          • Level6 Alpha amino acids and derivatives
            • Level7 Cysteine and derivatives

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Clase: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Amino acids and derivatives - Alpha amino acids and derivatives
• Direct Parent: Cysteine and derivatives
• Alternative Parents: Benzothiazoles 1-hydroxy-2-unsubstituted benzenoids Imidothiolactones Thiazolines Thiazoles Heteroaromatic compounds Carboxylic acid salts Sulfenyl compounds Propargyl-type 1,3-dipolar organic compounds Organic alkali metal salts Monocarboxylic acids and derivatives Imidothioic acids and derivatives Azacyclic compounds Organopnictogen compounds Organooxygen compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Cysteine or derivatives - 1,3-benzothiazole - 1-hydroxy-2-unsubstituted benzenoid - Imidothiolactone - Benzenoid - Heteroaromatic compound - Meta-thiazoline - Thiazole - Azole - Imidothioester - Carboxylic acid salt - Azacycle - Organic alkali metal salt - Organic metal salt - Organoheterocyclic compound - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Sulfenyl compound - Monocarboxylic acid or derivatives - Imidothioic acid or derivatives - Organic nitrogen compound - Organic oxygen compound - Organopnictogen compound - Organic oxide - Hydrocarbon derivative - Organic salt - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Aromatic heteropolycyclic compound
• Descripción: This compound belongs to the class of organic compounds known as cysteine and derivatives. These are compounds containing cysteine or a derivative thereof resulting from reaction of cysteine at the amino group or the carboxy group, or from the replacement of any hydrogen of glycine by a heteroatom.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Propiedades químicas y físicas

• Solubilidad: No less than 40mg/mL in PBS or pure water
• Peso molecular: 302.300 g/mol
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 7
• Rotatable Bond Count: 2
• Exact Mass: 301.98 Da
• Monoisotopic Mass: 301.98 Da
• Topological Polar Surface Area: 139.000 Ų
• Heavy Atom Count: 19
• Formal Charge: 0
• Complexity: 396.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 1
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 2

XLogP3

Referencias

1. Xiao Kui, Zhang Niyuan, Li Feifei, Hou Dayong, Zhai Xiaoyi, Xu Wanhai, Wang Gelin, Wang Hao, Zhao Liang.  (2022)  Pro-oxidant response and accelerated ferroptosis caused by synergetic Au(I) release in hypercarbon-centered gold(I) cluster prodrugs.  Nature Communications,  13  (1): (1-13).  [PMID:35945240] [10.1038/s41467-022-32474-y]

2. Chao Chen, Hongxia Zhang, Lingling Qi, Haoqi Lei, Xuefei Feng, Yingjie Chen, Yuanyuan Cheng, Defeng Pang, Jufeng Wan, Haiying Xu, Shifeng Cao, Baofeng Yang, Yan Zhang, Xin Zhao.  (2025)  Obesity-driven oleoylcarnitine accumulation in tumor microenvironment promotes breast cancer metastasis-like phenotype.  Acta Pharmaceutica Sinica B,      [PMID:40486851] [10.1016/j.apsb.2025.02.026]

3. Song Zhengwei, Sun Quanwei, Yang Wenshuo, Li Yunlong, Hu Chaoyu, Chen Chen, Liu Kang, Shen Wei, Yang Ye, Yin Dengke.  (2025)  Inflammation-targeted nanomedicine prevents tumor metastasis following photodynamic therapy by reversing epithelial-mesenchymal transition and ROS-mediated immunosuppression.  JOURNAL OF NANOBIOTECHNOLOGY,  23  (1): (1-19).  [PMID:40186261] [10.1186/s12951-025-03332-y]

4. Dong-Ni Pei, Yang Song, Ying-Xia Zhou, Bo Shu, Shao-wei Huang, Fa-Zhao Li, Wei-Dong Dai, Bao-Ye Sun.  (2025)  Enhancing cDC1-mediated anti-tumor immunity limits tumor progression and potentiates anti-PD-1 therapy in intrahepatic cholangiocarcinoma.  Frontiers in Immunology,      [PMID:41459487] [10.3389/fimmu.2025.1708962]

5. Xianghua Zhong, Xinchao Liu, Jiajia Luo, Xinyang Liu, Xueting Wei, Xi Peng, Lu Wang, Huaimin Wang, Kunyu Zhang, Liming Bian, Peng Shi.  (2026)  Cell surface engineering with a pseudofibrotic ECM reprograms the antifibrotic activity of mesenchymal stromal cells.  Science Advances,  12  (3):   [PMID:41544160] [10.1126/sciadv.aea0998]