Identification of chordoma-associated genes and screening experiments
Chordoma-associated gene identification and screening assays for (1) molecular mechanisms of chordoma (2) gene diagnosis (3) gene therapy.
Tema: RNA Lab
Artículos por tema "RNA Lab"
Knowledge Base
Yeast Spliceosome Analysis Experiment
A precursor is a macromolecular ribonucleoprotein complex that splices mRNA precursors in the nucleus of eukaryotic cells. It consists of five snRNPs and a larg
Experimental detection of protein-double-stranded RNA interactions by methylene blue-mediated photocrosslinking reaction
Methylene blue-mediated photocrosslinking reactions are a complement to UV crosslinking reactions. Methylene blue belongs to the phenothiazine group of dyes. Du
Solubilization and preservation of RNA in formamide
Storing RNA in formamide has many advantages over storing it in water or ethanol: (1) formamide will protect the RNA from degradation by RNA enzymes, and RNA st
Biotinylated oligodeoxythymidine-streptavidin magnetic bead purification band Poly(A)+RNA
The use of magnetic microspheres instead of transferring fiber cords as a medium is more commonly used and has been commercialized.
Experimental purification and removal of nucleoprotein complexes by antisense affinity chromatography
This experiment was derived from "RNA Laboratory Guidebook", edited by Xiaofei Zheng.
Oligodeoxythymidine cellulose purification band poly( A)+RNA
Most mammalian messenger RNAs (mRNAs) carry a poly(A)+ tail, and Poly(A)+-carrying RNAs can be isolated from total RNA using this protocol.
Chromosome preparation experiments on cultured peripheral blood cells
Although chromosomes can be prepared from other cells, human peripheral blood leukocytes are the easiest to synchronize, allowing high-resolution analysis of th
In situ hybridization experiments on paraffin sections or cells
In situ hybridization experiments for cellular RNA are used to localize specific mRNAs in mixed cell populations and tissues.