3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
2-Mercaptoethylamine•HCl (2-MEA) is a mild reducing agent suitable for selectively cleaving hinge-region disulfide bonds of IgG heavy chains. Several popular methods for conjugation or immobilization of antibodies involve covalent attachment specifically through sulfhydryl (−SH) groups.
Dithiothreitol is the trans isomer of the compound 2,3-dihydroxy-1,4-dithiolbutane. DTT, also known as Cleland’s Reagent, is a cell-permeable, strong but mild reducing agent, with a low redox potential of -0.33 V at pH 7 (optimal pH range 7.1 to 8.0).
TCEP is a potent, versatile, odorless, thiol-free reducing agent with broad application to protein and other research involving reduction of disulfide bonds. The unique compound is easily soluble and very stable in many aqueous solutions. TCEP reduces disulfide bonds as effectively as ...
Aminooxy PEG Linkers can be used in bioconjugation through the reaction of the aldehyde group with aminooxy to form aldoxime. It is much more stable than hydrazone and imine. In most of cases, it can be used directly without reduction while hydrazine and imines bond normally need be reduced to ...
PEG Ald reagents can be used in bioconjugation through the reaction of the aldehyde group with aminooxy to form aldoxime. It is much more stable than hydrazone and imine. In most of cases, it can be used directly without reduction while hydrazine and imines bond normally need be reduced to form ...
SPDP-PEG crosslinkers enable protein conjugations via amine-to-amine or amine-to-sulfhydryl crosslink. The polyethylene glycol (PEG) spacer arms confer great solubility in the reaction media. SPDP-PEG crosslinkers are best dissolved in organic solvent to make the stock solution. before adding ...
The PEG PFP Ester is a class of PEG labeling reagents that react with primary and secondary amines primary amines to form amide bonds. The pentafluorophenyl (PFP) ester is less subject to hydrolysis than NHS esters, resulting in more efficient reactions. PEG PFP Ester must first be dissolved in ...
Thiol PEG is a thiol-terminated polyethylene glycol (PEG)-containing reagent with either a methyl ether, hydroxyl or carboxylic acid group at the other end. These reagents have defined molecular weights and spacer lengths and are used for modifying surfaces such quantum dots, self-assembled ...
PEG Maleimide reagents