Electrical activity of single neurons: the membrane clamp technique
Over the last 20 years, the membrane clamp technique has become one of the main research tools in modern electrophysiology. Initially used only to measure singl
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Central nervous system culture techniques: hands-on experiments in chick embryo neuron culture
Establishing an accurate and reusable neuronal culture model in vitro is of self-evident importance for experimental research in neuroscience. Many research gro
High-throughput holistic in situ hybridization for the detection of environmental disturbances in zebrafish (Danio rerio), an embryonic tissue-specific gene expression alteration experiment
Holistic in situ hybridization is a process that allows observation of cellular gene expression (mRNA) in intact organisms. By comparing differences in gene exp
In vitro reconstruction of neuronal loops: experiments on methods for building a simple model system
We can reconstruct the S-cell network that initiates the basic respiratory behavior on cultured cells of the freshwater mollusc phylum Cnidaria by using in vitr
Laboratory methods for the detection of chromosomal structural aberrations in human and mouse spermatozoa by fluorescence in situ hybridization
This new generation of sperm F I S H assays has identified multiple paternal risk factors such as age, polypharmacy, lifestyle, and environmental aspects and oc
Application of suppression subtractive hybridization to detect metagenomic diversity in environmental samples
Metagenomics provides information on the total genetic structure and functional composition of nondisposing samples of microbial environments without the need t
Optical recording experiments of population neurons in brain slices
Optical recording involves the use of molecular indicators whose optical properties (absorption or fluorescence) vary according to the parameters of cellular ac
Experimental molecular haplotype analysis method based on emulsion polymerase chain reaction
Genotypes can be assayed by a variety of experimental methods, but assays for haplotype analysis, such as molecular haplotype analysis, are limited. Dependent o
Experiments on the resolution of electromyographic signals in muscle fibers
The precise EMG analysis techniques covered in this chapter recover all available information in the EMG signal.EMG signal analysis consists of two categories: