Cyclodextrin-containing polycations for nucleic acid delivery
A large number of non-viral systems have been used for nucleic acid delivery to cultured cells in vitro or to specific cells in vivo. They vary considerably in
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Recombinant herpes simplex virus vector
The natural physiological characteristics of H S V -I include the establishment of a lifelong latent state in neurons, where the viral gene remains a free molec
Polylysine copolymers for gene delivery
Polylysine and its copolymers have gained wide application as non-viral gene vectors. Although polylysine itself is toxic to cells, the covalent attachment of p
Preservation and amplification of targeted modified measles viruses
The S T A R System (6 Histidine Labeling and Retargeting) employs a pseudo-receptor and a label for a virus to enable the rescue and amplification of fully reco
Down-regulation of genes generated by silencing lentiviral vectors in mice.
This chapter describes the use of lentiviral vectors to transfer genes and small interfering RNA (siR N A ) expression cassettes to preimplantation crystalline
Experiments on mifepristone-induced gene regulatory systems
The mifepristone-induced gene regulatory system (M I G R S ) is a system for regulating rake gene expression through the antiprogesterone drug, mifepristone (M
Non-viral gene transfer experiments with Trojan horse liposomes that can cross the blood-brain barrier
Non-viral vector plasmid DNA can be delivered to the human brain via the vascular route across the blood-brain barrier by intravenous injection of DNA encapsula
Experiments with biodegradable nanoparticles
Biodegradable nanoparticles are colloidal particles, typically around IOOnm in diameter, which can be prepared from biodegradable polymers such as polylactic ac
Experiments on the isolation of subcellular organelles and subcellular structures
In proteomics research, a key step in all enrichment techniques is the subcellular fractionation, which is particularly important for the analysis of intracellu