3,200+ step-by-step lab protocols and SOPs for cell culture, staining, PCR, protein work and microbiology, with reagents, conditions and evaluation criteria.
The cellular wound healing assay is widely used and improved by researchers to study the role of various experimental conditions such as gene knockout and chemo
Analysis against nucleic acid sequences is the process of searching for genes in nucleic acid sequences, identifying the location of genes and the location of f
Determination of chlorophyll content can be used to: (1) determine the photosynthetic capacity, i.e., health, of the plant; (2) as data for research to improve
In vivo staining can be used to (1) show certain natural structures within the cell using certain non-toxic or minimally toxic dyes, and (2) not affect the life
Prokaryotic genome assembly and sequencing has a mature methodology, but there is no fast and easy way to annotate the assembled genome.
Prokka: rapid prokaryot
Tissue homogenization followed by suspension in isotonic media for differential centrifugation is a common method for separating organelles. The settling rate o
Mitosis, also known as indirect division, was first discovered in animals by W. Fleming (1882) and in plants by E. Strasburger (1880). Characterized by the pres
The preparation of plant protoplasts can be used for: (1) due to its ability to regenerate cell walls, carry out successive cell divisions and regenerate comple
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