Step-by-step protocols for preparing and using β-Nicotinamide adenine dinucleotide (NAD) in enzymatic assays, redox studies, and cell-free systems.
Topic
Enzymatic Assay
Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
5 resources · protocols, FAQs & articles
Step-by-step protocol for measuring creatinine in serum, plasma, and urine using Jaffe colorimetric and enzymatic assay methods for research labs.
β-Glucan is a class of polysaccharides formed by glucose residues linked through β-glycosidic bonds. It is widely present in oats, barley, yeast, fungi, algae and some microbial-derived materials. β-Glucans from different sources vary greatly in linkage type, branching degree, molecular ...
Glycerol and triglycerides (TG) are two important analytical targets in lipid metabolism research, biochemical testing, food analysis, and fermentation process monitoring. Glycerol is not only the structural backbone of triacylglycerols, phospholipids, and related lipid molecules, but also a key ...
Glucose-6-phosphate dehydrogenase (G6PD) is the key rate-limiting enzyme of the oxidative phase of the pentose phosphate pathway (PPP). It catalyzes the oxidation of glucose-6-phosphate (G6P) to 6-phosphoglucono-δ-lactone while reducing NADP⁺ to NADPH. NADPH is pivotal for antioxidant ...
Related topics
Techniques often explored alongside immunological experiments.