1,700+ technical articles by Aladdin Scientific's scientists on reagent selection, reaction mechanisms, assay design and formulation. New every week.
Poly(ethylene glycol) (PEG) is a synthetic, hydrophilic, biocompatible polymer with widespread use in biomedical and other applications. PEGs are synthesized using a ring-opening polymerization of ethylene oxide to produce a broad range of molecular weights and molecular weight distributions ...
α-Amylase (α-amylase) is a class of glycoside hydrolases widely distributed in animals, plants, and microorganisms. Acting in an endo-fashion, it catalyzes cleavage of α-1,4-glycosidic bonds in starch, glycogen, and related α-1,4-glucans, generating products such as maltose, maltotriose, and ...
Molecular cloning screening and identification are used to confirm whether the target insert has been correctly introduced into the vector, whether the insertion orientation matches the design, whether the reading frame is continuous, whether sequence mutations are present, and whether the ...
Lysosomes are core organelles for cellular catabolism and quality control. They are responsible for degrading materials derived from intracellular autophagy, extracellular endocytosis, phagocytosis, and macropinocytosis. In addition to degradation, lysosomes participate in nutrient sensing, mTOR ...
Platelet-derived growth factor (PDGF) is a class of disulfide-linked dimeric growth factors. It was originally named because it is present in platelet α-granules and is released after platelet activation. PDGF can promote the proliferation, migration, and chemotaxis of smooth muscle cells, ...
Mitochondrial functional disturbance can activate a series of stress signals to restore energy metabolism, protein homeostasis, and redox balance. Decreased membrane potential, impaired respiratory chain function, defective protein import, or abnormal mitochondrial proteostasis can induce ...
Cell permeabilization is performed after cell fixation to open membrane structures, allowing antibodies, dyes, or probes to enter the cytoplasm, nucleus, or organelles and recognize target antigens. This step directly affects localization accuracy in immunofluorescence and also influences ...
Lipid droplets (LDs) are dynamic organelles formed by a phospholipid monolayer surrounding a neutral lipid core. Their core components mainly include triacylglycerol (TAG) and cholesteryl esters. Lipid droplets are not only lipid storage structures, but also participate in energy metabolism, ...
Bacterial cell wall degradation is a key technique that disrupts peptidoglycan and its outer structures through enzymatic, chemical, or physically assisted methods, allowing cellular contents to be released or enabling the formation of protoplasts/spheroplasts. This technology is commonly used ...