ExactAb™, Validated, Carrier Free, 1.0 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Mouse anti HA tag Antibody, Monoclonal (4G3), could be used for WB, IF, ICC and so on.
Application:
WB: 1/1000 - 1/2000
IF/ICC: 1/1000
Relevance
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
HA tag Mouse mAb (Ab106776) - Western Blot
All lanes: HA tag Mouse mAb (Ab106776) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/50000 dilution
Predicted band size: 75 kDa
Observed band size: 77 kDa
Exposure time: 30.0 s
HA tag Mouse mAb (Ab106776) - Western Blot
All lanes: HA tag Mouse mAb (Ab106776) at 1/1000 dilution
Samples: Recombinant Human HA-Ubiquitin Protein (rp170435) at 20 ng per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/30000 dilution
Predicted band size: 11 kDa
Observed band size: 10 kDa
Exposure time: 30.0 s
HA tag Mouse mAb (Ab106776) - IF/ICC
IF image of HA tag Mouse mAb (Ab106776) stained HEK293T cells, transfected with pcDNA3.1-HA-Flag-p62. The cells were 4% para-formaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1 h to permeabilise the cells and block non-specific pro-tein-protein interactions. The cells were then incubated with the HA tag Mouse mAb (Ab106776) (1/200) and the antibody Flag-tag pAb(1/200) at 5 µg/mL overnight at 4°C. The secondary anti-body (Red) was Goat anti-Mouse IgG (H+L)-TRITC and the secondary antibody (Green) was Goat anti-Rabbit IgG (H+L)-FITC used at 1/1000 dilution for 1 h. Hoechst33342 was used to stain the cell nuclei (blue).
| Aplicación | Dilution info |
|---|---|
| WB | 1/1000 - 1/2000 |
| IF/ICC | 1/1000 |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ23F1101592 | Certificate of Analysis | Feb 10, 2026 | Ab106776 |
| ZJ23F1101593 | Certificate of Analysis | Feb 10, 2026 | Ab106776 |
| ZJ24F0505917 | Certificate of Analysis | May 21, 2024 | Ab106776 |
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