ExactAb™, Validated, Carrier Free, High performance, 1.0 mg/mL Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Mouse anti His tag Antibody, Monoclonal antibody(4C2), could be used for WB, IF, ICC and so on.
Application:
WB: 1/5000 - 1/10000
IF/ICC: 1/50 - 1/200
Relevance
The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. His-tags can be fused to the amino- or carboxy- termini of proteins in transfected or transformed cells.
His tag Mouse mAb (Ab086831) - Western Blot
All lanes: His tag Mouse mAb (Ab086831) at 1/4000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 116 kDa
Observed band size: 47, 53,112 kDa
Exposure time: 21.9 s
His tag Mouse mAb (Ab086831) - IF/ICC
IF image of His tag Mouse mAb (Ab086831) stained HEK293F cells, transfected with pcDNA3.1-TfR-hFC-His. The cells were 4% paraformaldehyde fixed (15 min) and then incubated in 10% normal goat serum for 1h to per-meabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with His tag Mouse mAb (Ab086831) (1/50) overnight at 4°C. The secondary antibody (Green) was Goat anti-Mouse IgG (H+L)-FITC. Hoechst33342 was used to stain the cell nuclei (blue).
His tag Mouse mAb (Ab086831) - IF/ICC
IF image of His tag Mouse mAb (Ab086831) stained HEK293F cells, transfected with pcDNA3.1-His-TfR-hFC. The cells were 4% paraformaldehyde fixed (15 min) and then incubated in 10% normal goat serum for 1h to per-meabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with His tag Mouse mAb (Ab086831) (1/50) overnight at 4°C. The secondary antibody (Green) was Goat anti-Mouse IgG (H+L)-FITC. Hoechst33342 was used to stain the cell nuclei (blue).
| Aplicación | Dilution info |
|---|---|
| IF/ICC | 1/50 - 1/200 |
| WB |
1/5000 – 1/10000
|
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Find and download the COA for your product by matching the lot number on the packaging.
| Lot Number | Certificate Type | Fecha | Articulo |
|---|---|---|---|
 ZJ23F1202137 | Certificate of Analysis | Apr 13, 2026 | Ab086831 |
| ZJ23F1202138 | Certificate of Analysis | Apr 13, 2026 | Ab086831 |
| ZJ24F0404257 | Certificate of Analysis | Apr 09, 2024 | Ab086831 |
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