L-Glutamic Acid Solution (0.2 mol/L, sterile) - sterile-filtered,BioReagent,for cell culture,0.2 mol/L , CAS No.56-86-0

CAS: 56-86-0 Cat. No.: L1509602 Peso molecular: 147.13 Beilstein Registry Number: 1723801 Número EC: 200-293-7

GRADE & PURITY Sterile-filtered, BioReagent, for Cell culture, 0.2 mol/L

Synonyms

L-Glutamic Acid Solution

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Application

Cell Culture

Descripción general

  L-Glutamic acid has a molecular weight of 147.13, a molecular formula of C₅H₉NO₄, and a CAS number of 56-86-0. It is a type of acidic amino acid, containing two carboxyl groups in its molecule, and its chemical name is α-aminoglutaric acid. First discovered by Ritthausen in 1856, glutamic acid appears as colorless crystals with a savory taste. It is slightly soluble in cold water, readily soluble in hot water, and soluble in hydrochloric acid solution, with an isoelectric point of 3.22.

  Glutamic acid is abundant in cereal proteins and also present in high concentrations in animal brains. It plays a crucial role in protein metabolism in organisms and participates in numerous important chemical reactions in animals, plants, and microorganisms. It serves as a raw material for producing monosodium glutamate (MSG) and fragrances, and can also be used as an additive in culture media for mammalian cell culture, boasting extremely wide applications.

  The L-Glutamic Acid Solution (0.2 mol/L) is mainly composed of L-glutamic acid (approximately 2.9%) and deionized water, and is sterilized by filtration. This reagent is for research use only and is not intended for clinical diagnosis or any other purposes.

Operating Procedures (For Reference Only)

1. After removing the product from the low-temperature refrigerator, allow it to thaw naturally at room temperature first. The presence of white insoluble substances is a normal phenomenon. The solution can be placed in a water bath at 60-65°C to assist dissolution; it will fully redissolve into a completely transparent state within 45-60 minutes. Do not exceed 75°C in the water bath, as this may cause deformation of the bottle.

2. Operate according to the specific requirements of the experiment. Generally, it is used at a ratio of culture medium: L-Glutamic Acid Solution=100: 1.

Precautions

1. Avoid repeated freezing and thawing, and ensure aseptic operation during use. After the first dissolution, it is recommended to aliquot the solution into small portions and store them at low temperature.

2. L-Glutamic acid is a white crystalline powder with almost no odor. It decomposes at 224-225°C, and the pH value of its saturated aqueous solution is approximately 3.2. It is sparingly soluble in water, practically insoluble in ethanol and ether, and highly soluble in formic acid. Its solubility in water (g/100g water) is as follows: 0.72 (20°C), 1.51 (40°C), 3.17 (60°C), and 6.66 (80°C).

3. Please use the reagent as soon as possible after opening to prevent any adverse effects on subsequent experimental results.

4. For your safety and health, wear a lab coat and disposable gloves during operation.

Specifications

Sinónimos

L-Glutamic Acid Solution

Especificaciones y pureza

sterile-filtered, BioReagent, for cell culture, 0.2 mol/L

Estabilidad y almacenamiento

Store at -20℃ long term (12 months).

Condiciones de almacenamiento de almacenamiento

Store at -20°C

Enviado en

Ice chest + Ice pads

Este producto requiere envío en cadena de frío. Los servicios terrestres y otros servicios económicos no están disponibles.

Grado

BioReagent, for Cell culture, Sterile-filtered

Nombres e identificadores

• Isómeros SMILES: C(CC(=O)O)[C@@H](C(=O)O)N
• WGK Alemania: 1
• Peso molecular: 147.13
• Beilstein: 1723801
• Reaxy-Rn: 1723799
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1723799&ln=

Propiedades químicas y físicas

• Rotación específica [α]: 32 ° (C=5, HCl)
• Punto de fusión (°C): 205°C

Referencias

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