Recombinant MHC Class II Antibody (AF405) - Primary antibody, specific to MHC Class II

Cat. No.: Ab213991
Disponible para pedir
GRADE & PURITY 0.5 mg/mL
Application
Flow Cytometry
Host species
Rat
Reactive species
Mouse
Isotype
Rat IgG2b
Clone Number
P24J10
Conjugation
AF405
 ·  off list, applied to all prices below.
Size
Estado
Price
Qty
10μg
Ab213991-10μg
4
75,90US$
25μg
Ab213991-25μg
3
151,90US$
100μg
Ab213991-100μg
1
377,90US$
Enter a quantity for the sizes you want to add.
🧪

Why this grade

0.5 mg/mL for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Descripción general

Recombinant MHC Class II Antibody, clone P24J10, could be used for Flow Cytometry.

Specifications

Product Name
Recombinant MHC Class II Antibody (AF405) - Primary antibody, specific to MHC Class II
Sinónimos
D6S221E antibody | DMA antibody | DMB antibody | DP beta 1 antibody | DP beta 1 chain antibody | DP(W4) beta chain antibody | DPB 1 antibody | DPB1 antibody | DPB1_HUMAN antibody | DRB antibody | H2Ea antibody | HLA class II histocompatibility antigen ant
Especificaciones y pureza
0.5 mg/mL
Mecanismos bioquímicos y fisiológicos
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides
Especies hospedadoras
Rat
Especificidad
MHC Class II
Inmunógeno
Activated C57BL/6 mouse spleen cells
Control positivo
Flow: C57BL/6 mouse splenocytes
Conjugación
AF405
Excitación (nm)
Violet Laser(405nm)
Emisión (nm)
422nm
Ex/Em(nm)
Ex:401nm;Em:422nm
Tipo de cadena ligera
kappa
Método de purificación
Protein A purified
Concentración
0.5 mg/mL
Almacenamiento y envío
Condiciones de almacenamiento de almacenamiento
Store at 2-8°C,Protected from light,Do not freeze
Enviado en
Wet ice,Do not freeze
Estabilidad y almacenamiento
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Imágenes

Recombinant MHC Class II Antibody (AF405) (Ab213991) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with Recombinant MHC Class II Antibody (AF405) (Ab213991) at 0.2μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF405) (Ab190070).

Recombinant MHC Class II Antibody (AF405) (Ab213991) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with Recombinant MHC Class II Antibody (AF405) (Ab213991) at 0.2μg/test for 1 hour at 4°C. Blue - Rat IgG (AF405) (Ab190070). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación
AplicaciónDilution info
Flow Cytometry

≤0.3μg/test

For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Objetivos asociados (humanos)
HLA-DPB1 Tbio HLA class II histocompatibility antigen, DP beta 1 chain (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificados (CoA, COO, BSE/TSE y tabla de análisis)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Preguntas frecuentes y artículos
Calculadoras de soluciones
Reseñas

Reseñas de cliente

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