Recombinant MHC Class II Antibody (PE) - Primary antibody, specific to MHC Class II

Cat. No.: Ab213998

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2b

Clone Number

P24J10

Conjugation

PE

Descripción general

Recombinant MHC Class II Antibody, clone P24J10, could be used for Flow Cytometry.

Specifications

Product Name

Recombinant MHC Class II Antibody (PE) - Primary antibody, specific to MHC Class II

Sinónimos

D6S221E antibody | DMA antibody | DMB antibody | DP beta 1 antibody | DP beta 1 chain antibody | DP(W4) beta chain antibody | DPB 1 antibody | DPB1 antibody | DPB1_HUMAN antibody | DRB antibody | H2Ea antibody | HLA class II histocompatibility antigen ant

Especificaciones y pureza

ExactAb™, Validated, 0.5 mg/mL

Mecanismos bioquímicos y fisiológicos

Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides

Especies hospedadoras

Rat

Especificidad

MHC Class II

Inmunógeno

Activated C57BL/6 mouse spleen cells

Control positivo

Flow: C57BL/6 mouse splenocytes

Conjugación

PE

Excitación (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emisión (nm)

575nm

Ex/Em(nm)

Ex:565nm;Em:575nm

Grado

ExactAb™, Validated

Tipo de cadena ligera

kappa

Método de purificación

Protein A purified

Concentración

0.5 mg/mL

Almacenamiento y envío

Condiciones de almacenamiento de almacenamiento

Store at 2-8°C,Protected from light,Do not freeze

Enviado en

Wet ice,Do not freeze

Estabilidad y almacenamiento

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Imágenes

Flow Cytometry: Recombinant MHC Class II Antibody (PE) (Ab213998)

Recombinant MHC Class II Antibody (PE) (Ab213998) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant MHC Class II Antibody (PE) (Ab213998) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE) (Ab180312).

Flow Cytometry: Recombinant MHC Class II Antibody (PE) (Ab213998)

Recombinant MHC Class II Antibody (PE) (Ab213998) - Flow Cytometry
Flow cytometry analysis of MHC Class II expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). C57BL/6 mouse splenocytes cells were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with Recombinant MHC Class II Antibody (PE) (Ab213998) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (PE) (Ab180312). Black - Unlabelled control, cells without incubation with primary antibody.

Aplicación

• Aplicación: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Objetivos asociados (humanos)

HLA-DPB1 Tbio HLA class II histocompatibility antigen, DP beta 1 chain (0 Activities)

Discover Target: HLA-DPB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)