Imidazole - Suitable for molecular biology, ≥99.5% , CAS No.288-32-4

CAS: 288-32-4 Cat. No.: I108706 分子式: C₃H₄N₂ 分子量: 68.08 Beilstein Registry Number: 103853 EC番号: 206-019-2

グレード＆純度 Suitable for molecular biology, ≥99.5%

Synonyms

Glyoxaline | 1,3-Diaza-2,4-cyclopentadiene | Glyoxaline | 3-Azapyrrole

Storage

Room temperature,Argon charged,Desiccated

Shipped In

Normal

概要

Imidazole, also known as Glyoxalin, is an aromatic heterocyclic diazole and a key biological buffer with a pH range of 6.2 to 7.8 at 25°C. It has a wide range of applications in molecular biology, biochemistry, and proteomics. In proteomics, imidazole is essential for eluting histidine-tagged proteins from IMAC columns. It is a strong chelator of divalent cations, such as nickel and zinc, which disrupts the interaction between the His-tag and the metal ions. This allows for the efficient elution of purified proteins.

Furthermore, imidazole is indispensable for enhancing protein purity. It is widely used in preparing buffer solutions optimized for downstream purification columns. Imidazole solutions are also effective elution and washing agents throughout protein purification processes. Additionally, imidazole can be used for assays of horseradish peroxidase (HRP) and reverse staining of SDS-PAGE gels for protein detection. Imidazole is a versatile and valuable reagent for a wide range of biological applications. It is an essential component of many buffers and is also used in protein purification and detection protocols.

仕様

同義語

Glyoxaline | 1,3-Diaza-2,4-cyclopentadiene | Glyoxaline | 3-Azapyrrole

仕様と純度

Suitable for molecular biology, ≥99.5%

生化学的・生理学的メカニズム

Imidazole derivatives have antibacterial, antifungal and anticancer functionality. It interacts with DNA and also binds to protein and stops cell division. It also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 F

保管条件

Room temperature,Argon charged,Desiccated

入荷

Normal

等級

Suitable for molecular biology

純度

≥99.5%

名前と識別子

パブケム・シド

508812952

パブケム・シド・ウル

https://pubchem.ncbi.nlm.nih.gov/substance/508812952

カノニカル・スマイル

C1=CN=CN1

IUPAC Name

1H-imidazole

InChIKey

RAXXELZNTBOGNW-UHFFFAOYSA-N

INCHI

1S/C3H4N2/c1-2-5-3-4-1/h1-3H,(H,4,5)

異性体SMILES

C1=CN=CN1

WGKドイツ

1

RTECS

NI3325000

UN番号

2923

分子量

68.08

Beilstein

103853

Reaxy-Rn

103853

Reaxys-RN_link_address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=103853&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • 分類 Azoles
      • Subclass Imidazoles

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• 分類: Azoles
• Subclass: Imidazoles
• Intermediate Tree Nodes: Not available
• Direct Parent: Imidazoles
• Alternative Parents: Heteroaromatic compounds Azacyclic compounds Organopnictogen compounds Organonitrogen compounds Hydrocarbon derivatives
• Molecular Framework: Aromatic heteromonocyclic compounds
• Substituents: Heteroaromatic compound - Imidazole - Azacycle - Organic nitrogen compound - Organopnictogen compound - Hydrocarbon derivative - Organonitrogen compound - Aromatic heteromonocyclic compound
• 説明: This compound belongs to the class of organic compounds known as imidazoles. These are compounds containing an imidazole ring, which is an aromatic five-member ring with two nitrogen atoms at positions 1 and 3, and three carbon atoms.
• External Descriptors: an aromatic compound

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

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ALDH1A1 Tchem Aldehyde dehydrogenase 1A1 (77053 Activities)

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HL-60 (67320 Activities)

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SMMC-7721 (5516 Activities)

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関連ターゲット（非人間）

Nos2 Nitric oxide synthase, inducible (3573 Activities)

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CHO (4503 Activities)

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Rorc Nuclear receptor ROR-gamma (89407 Activities)

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作用メカニズム

化学的性質と物理的性質

• 感度: Moisture sensitive，air sensitive
• 引火点（°C）: 145℃
• 沸点（°C）: 257°C
• 融点（°C）: 90-91°C
• 分子量: 68.080 g/mol
• XLogP3: -0.100
• Hydrogen Bond Donor Count: 1
• Hydrogen Bond Acceptor Count: 1
• Rotatable Bond Count: 0
• Exact Mass: 68.0374 Da
• Monoisotopic Mass: 68.0374 Da
• Topological Polar Surface Area: 28.700 Ų
• Heavy Atom Count: 5
• Formal Charge: 0
• Complexity: 28.100
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1