CEBPA Mouse mAb - 1차 항체, specific to CEBPA, IgG1

Cat. No.: Ab095961

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0mg/mL

Application

ELISA, Flow Cytometry, WB

Host species

마우스

Reactive species

인간

Isotype

Mouse IgG1

Clone Number

4E10

Conjugation

Unconjugated

개요

Mouse Monoclonal (4E10) to CEBPA , could be used for WB, Flow and ELISA.

Applications
WB：1/500 - 1/2000;
Flow：1/200 - 1/400;
ELISA ：1/10000;

Purity
Protein G purified

Function
C/EBP is a DNA-binding protein that recognizes two different motifs: the CCAAT homology common to many promoters and the enhanced core homology common to many enhancers.

Specifications

Product Name

CEBPA Mouse mAb - 1차 항체, specific to CEBPA, IgG1

동의어

C/EBP alpha

사양 및 순도

ExactAb™, Validated, Carrier Free, 1.0mg/mL

숙주 종

마우스

특이성

CEBPA

면역원

Purified recombinant fragment of human CEBPA expressed in E. Coli.

포지티브 제어

WB: THP-1 cell lysate, Flow: HeLa cells.

변화

Unconjugated

등급

Carrier Free, ExactAb™, Validated

아이소타입

IgG1

라이트 체인 유형

kappa

SDS-PAGE

150 kDa

정화 방법

Protein G purified

집중력

1.0mg/mL

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C. Avoid freeze/thaw cycle.

이미지

Western Blot: CEBPA Mouse mAb (Ab095961)

CEBPA Mouse mAb (Ab095961) - Western Blot
All lanes: CEBPA Mouse mAb (Ab095961) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 42&26 kDa
Observed band size: 42&26 kDa

Flow Cytometry: CEBPA Mouse mAb (Ab095961)

CEBPA Mouse mAb (Ab095961) - Flow Cytometry
Flow cytometric analysis of HeLa cells using CEBPA Mouse mAb (Ab095961) at 1/200 dilution (green) and negative control (red).

적용

• 적용: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/200-1/400 ;

• ELISA:

  1/10000 ;

관련 대상(인간)

CEBPA Tbio CCAAT/enhancer-binding protein alpha (0 Activities)

Discover Target: CEBPA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

자주 묻는 질문

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CEBPA Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.