DUSP23 Mouse mAb - 1차 항체, specific to DUSP23

Cat. No.: Ab100591

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

마우스

Reactive species

마우스, 인간, 조언

Isotype

Mouse IgG2a

Clone Number

OTI3C10

Conjugation

Unconjugated

개요

Mouse anti Human DUSP23 Antibody, Monoclonal (OTI3C10), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

DUSP23 Mouse mAb - 1차 항체, specific to DUSP23

동의어

Dual specificity phosphatase 23 | Dual specificity protein phosphatase 23 | DUS23_HUMAN | Dusp23 | DUSP25 | EC 3.1.3.16 | EC 3.1.3.48 | FLJ20442 | LDP 3 | LDP-3 | LDP3 | Low molecular mass dual specificity phosphatase 3 | MOSP | RP11-190A12.1 | VH1 like m

사양 및 순도

ExactAb™, Validated, See COA

생화학 및 생리적 메커니즘

Protein phosphatase that mediates dephosphorylation of proteins phosphorylated on Tyr and Ser/Thr residues. In vitro, it can dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) in vitro. Able to enhance activation of JNK and p38 (MAPK14).

숙주 종

마우스

특이성

DUSP23

면역원

Recombinant full length protein of human DUSP23 produced in HEK293T.

포지티브 제어

WB: A549 and MCF7 cell lysates. IHC: Human breast, kidney, carcinoma of kidney, adenocarcinoma of ovary, pancreas, endometrium, adenocarcinoma of endometrium, prostate, bladder, tonsil tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY D

변화

Unconjugated

등급

ExactAb™, Validated

SDS-PAGE

150 kDa

정화 방법

Protein A purified

집중력

See COA

보관 및 배송

모양

Liquid

보관 조건

Store at -20°C,Avoid repeated freezing and thawing

배송

Ice chest + Ice pads

안정성 및 스토리지

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

이미지

Western Blot: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - Western Blot
All lanes: DUSP23 Mouse mAb (Ab100591) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/40000 dilution

Predicted band size: 16 kDa
Observed band size: 16 kDa
Exposure time: 66.0 s

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human breast tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human Kidney tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of Human kidney tissue using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human ovary tissue using using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human endometrium tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human endometrium tissue using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of Human bladder tissue using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human prostate tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IHC
Immunohistochemical staining of paraffinembedded Human tonsil tissue within the normal limits using DUSP23 Mouse mAb (Ab100591). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IF/ICC: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - IF/ICC
DUSP23 Mouse mAb (Ab100591) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DUSP23.

Flow Cytometry: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - Flow Cytometry
Flow cytometric Analysis of HeLa cells, using DUSP23 Mouse mAb (Ab100591) (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry: DUSP23 Mouse mAb (Ab100591)

DUSP23 Mouse mAb (Ab100591) - Flow Cytometry
Flow cytometric Analysis of Jurkat cells, using DUSP23 Mouse mAb (Ab100591) (Red), compared to a nonspecific negative control antibody (Blue).

적용

• 적용: Dilution info
• WB:

  1/2000;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

• IHC:

  1/150

관련 대상(인간)

DUSP23 Tbio Dual specificity protein phosphatase 23 (0 Activities)

Discover Target: DUSP23

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)