Experimental methods of Agrobacterium-mediated transformation of barley
Agrobacterium-mediated transformation of barley has been widely utilized over the last decade and it offers advantages over gene gun-mediated transgenic techniq
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Substantial equivalence (metabolomics) experiments
Modern "metabolomics" methods can be used to automatically compare the levels of a number of structurally different compounds in a large number of samples. The
Gene insertion site and pattern experiments
The study of insertion patterns and loci of exogenous genes in transgenic plants greatly contributes to the understanding of the mechanisms of integration, expr
Experiments on the design and management of field trials of transgenic crops
With the development of transgenic technology systems, the transfer of heterologous genes into the plant genome has become possible, which provides a new avenue
Substantial equivalence (proteomics) experiments
Wheat, as one of the major global crops, is processed into a range of food products for consumer consumption. Therefore, it is of great importance to determine
Substantial equivalence (transcriptomics) experiments
In Western Europe, for transgenic crops to be approved for commercial production, proof of their substantial equivalence to conventionally bred crops needs to b
Root meristem cell nuclear protein extraction experiment
The isolation and extraction of nuclear proteins has facilitated the study of plant proteomics. This technique relies on subcellular isolation with which the pr
Experimental proteomic analysis of Arabidopsis thaliana chloroplasts
This chapter describes a simple and practical method for large-scale isolation and purification of chloroplasts. The advantages of this method are high yield, l
Proteomic study of recalcitrant plant tissues (protein extraction by phenol method) Experiments
Another feasible method for protein extraction, besides the classical trichloroacetic acid (TCA ) /acetone precipitation method, is phenol extraction, which can