Plant whole protein extraction by trichloroacetic acid-acetone method
In this chapter we describe an efficient method suitable for whole protein extraction from various plant tissues. The method utilizes trichloroacetic acid and a
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Protein extraction experiments on woody plants
Within this chapter, we present a protocol for extracting total protein from wood-forming tissues (secondary xylem in differentiation). This protocol has been u
Xylem and phloem sap protein extraction experiments
It is well known that the xylem and primordial vasculature in higher plants are responsible for the long-distance transport of small nutrient molecules. However
Root meristem cell nuclear protein extraction experiment
The isolation and extraction of nuclear proteins has facilitated the study of plant proteomics. This technique relies on subcellular isolation with which the pr
Cereal plant seed protein extraction experiment
Seeds may contain different components, like starch and polysaccharides, which can severely reduce the amount of protein extracted. The proteins in cereal plant
Extraction and solubilization experiments of plant plasma membrane proteins
In all living cells the plasma membrane (PM ) exists as an interface between the cytoplasm and the environment and is one of the most complex and differentiated
Experiments on bidirectional electrophoresis techniques in plant proteomics
Bidirectional gel electrophoresis and mass spectrometry for protein identification using fixed pH gradients (IPGs ) are currently the main techniques for proteo
Experiments on the solubilization of proteins by descaling and leaving agents prior to bidirectional electrophoresis
Because of its outstanding ability to separate total proteins, bidirectional gel electrophoresis can be applied to the separation of all types of proteins. Howe
Experiments on the quantitative analysis of two-dimensional gels
Two-dimensional electrophoresis, as an important experimental tool for comparative proteomics studies, has been applied to conveniently reflect the differences