Analysis of mitogen-activated protein kinase (MAPK) activity in T cells
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
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Analysis of inositol phospholipid turnover during lymphocyte activation
Receptor-mediated activation of phospholipase C (P L C) induces hydrolysis of membrane inositol phospholipids to produce diacylglycerol (D A G) and water-solubl
Blotting assay for anti-phosphorylated tyrosine
Author: J.E. Colligan et al, Translator: Xuitao Cao et al, This experiment is from "Compendium Immunology Laboratory Guide".
Detection of tyrosine protein kinase by immunocomplex methods
The low abundance of tyrosine protein kinase (T P K ) expression and its rapid activation and inactivation make it difficult to purify using conventional bioche
Preparation of mouse T-cell hybrid tumors
T-cell hybridomas can be obtained by fusing activated T cells with tumor cells. Heterogeneous hybridomas can be cloned by limited dilution to obtain hybridomas
ELISPOT method for detection of cytokine-secreting cells
Enzyme-linked immunospot test (ELISPOT) is based on the principle that cells that secrete certain protein molecules (e.g. cytokines) are able to secrete a relat
Detection of IL-15
The ELISA method can be used to detect human IL-15 in culture supernatants and serum samples. This method can also be used for the detection of monkey IL-15, bu
Detection of IL-13
ELISA is highly specific for human or mouse IL-13 and does not recognize other cytokines in the sample. Author: J.E. Colligan et al, Translator: Xuitao Cao et a
IL-12 detection assay
This is a method for the specific detection of IL-12 heterodimers. Since IL-12 p4 0 monomer cannot be detected, this method is able to detect IL-12 p7 5 heterod